Protein Info for HP15_293 in Marinobacter adhaerens HP15

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 868 PF02739: 5_3_exonuc_N" amino acids 2 to 129 (128 residues), 148.9 bits, see alignment E=2.2e-47 TIGR00593: DNA polymerase I" amino acids 3 to 868 (866 residues), 917.1 bits, see alignment E=7.3e-280 PF01367: 5_3_exonuc" amino acids 130 to 224 (95 residues), 116.3 bits, see alignment E=1.5e-37 PF01612: DNA_pol_A_exo1" amino acids 270 to 456 (187 residues), 130.4 bits, see alignment E=1.3e-41 PF00476: DNA_pol_A" amino acids 491 to 866 (376 residues), 525.3 bits, see alignment E=1.8e-161

Best Hits

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PL15 at UniProt or InterPro

Protein Sequence (868 amino acids)

>HP15_293 DNA polymerase I (Marinobacter adhaerens HP15)
MISMLRRLEQDFPGSKMVVVFDAKGKTFRHDLYEEYKANRPPMPEDLACQIEPIHEIVKA
MGLPLLTVTGVEADDVIGTLANEATSKGIDVVVSTGDKDMAQLVSDHVTLINTMTETRMD
RDGVVEKFGVTPEQIVDYLALVGDKVDNIPGVNKCGPKTAVKWLQSYDNLDNVIEHADEI
KGKIGEYLRDATETLPLSRELATIRTDVELDFGLEDLKLREQDDQQLLELFREYEFRSWI
AELEEDESSSSSNARAGDETPKPETGEKDYSIITDQKELDQWLERLGAAELFAFDTETTS
LRYMDAEIVGVSFAVKPGEAAYVPLGHDYMGAPEQLDRDQVLDQLKPLLENPDLAKVGQN
LKYDKNVLANHGITLEGIAEDTMLESYVLNSVATRHDMDSLAMYYLGEKTTSFESIAGKG
AKQLTFNQIDLEKAAPYAAEDADITLRLHQTLRPKLKETGKLAAVYEDIDLPLVPVLSRM
EQRGTLISASTLRQHSQELAERMAELEKEAHEVAGETFNLGSTKQLQAIFYDKMGLPVIK
KPQRARRQQRSRCFRNSPTNTNCRVILEHRSLSKLKSTYTDTLPELIHHRTGRVHTSYHQ
AVTATGRLSSSEPNLQNIPIRSEQGRRIRQAFIAPEGYKLVAADYSQIELRIMAHLSGDK
GLLKAFEKGEDIHKATASEVFGVSLDDVSSDQRRSAKAINFGLIYGMSAFGLSRQLDVER
KLAQEYIDRYFERYPGVLKYMDNIRKQAHDDGFVETLFGRRLYLPEINARNKQLQQAAER
TAINAPMQGTAADIIKRAMVDVENWLLKEHADEARMTMQVHDELILEVKESALDKIRSGL
EKRMSAAAELDVPLLVEAGVGDNWDEAH