Protein Info for HP15_2883 in Marinobacter adhaerens HP15
Annotation: glutamyl-tRNA (Gln) amidotransferase subunit A
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01457, allophanate hydrolase [EC: 3.5.1.54] (inferred from 59% identity to pmy:Pmen_0704)Predicted SEED Role
"Allophanate hydrolase (EC 3.5.1.54)" in subsystem Urea decomposition (EC 3.5.1.54)
MetaCyc Pathways
- L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
- L-citrulline degradation (3/3 steps found)
- cyanate degradation (3/3 steps found)
- urea degradation I (3/3 steps found)
- cyanuric acid degradation II (3/5 steps found)
- superpathway of allantoin degradation in yeast (3/6 steps found)
- cyanuric acid degradation I (2/5 steps found)
- uracil degradation III (2/5 steps found)
- superpathway of atrazine degradation (3/8 steps found)
- allantoin degradation IV (anaerobic) (2/9 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.54
Use Curated BLAST to search for 3.5.1.54
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See E4PMD5 at UniProt or InterPro
Protein Sequence (604 amino acids)
>HP15_2883 glutamyl-tRNA (Gln) amidotransferase subunit A (Marinobacter adhaerens HP15) MLGWTIRDWQQAYKEGATPESLLGELLTGLDTTDVAWISLLDQKGLDEALAGLKQKLQDA GGEIDKLPLYGIPFAAKDNIDAVGFETTAACPAFAYTPEEDATTVARLKAAGAIVIGKTN LDQFATGLVGTRSPYGAVPNSFKPDVVSGGSSSGSASVVARGLAPFALGTDTAGSGRVPA GLNNLVGLKPTKGLFSIRGVVPACRSLDCVSIFALTVNDAGLVSDTMAGFDADDAFSRKP PYALPLDGPALRRPGPIQRLAIPDHPQWFGDQQAEAAWNTAISQWRQQNVELVPLDFSPM LELAALLYEGPWVAERHAAVESFMTSHGDDMNPVVKGIISKAGNFSATDTFKAQYRKEEL QRQIDELLADVDALLVPTAPTAPTIEAVNADPVALNSQLGTYTNFVNLADMSALAIPAGF RDDGLPFGVTLISGAWKDTELQRLACQWLNAHPTPLGATGNDRPEEAPGAAISTPTIKVA VVGAHLSGMPLNTQLSERCAVLLEQTTTSANYRLFALPNTTPPKPGLKRAAPGEGREIIV EVWEMPASAFGSFVDLIPAPLGIGNVELADGRWVNGFVCEGYGFEGAEDVTEFGGWRAYI ASRA