Protein Info for Psest_2930 in Pseudomonas stutzeri RCH2

Annotation: asparagine synthase (glutamine-hydrolyzing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03104: asparagine synthase family amidotransferase
  • amino acids 38 to 624 (587 residues), 970.4 bits, E=2.7e-296
• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 39 to 557 (519 residues), 402.8 bits, E=2.8e-124
• PF13522: GATase_6
  • amino acids 69 to 197 (129 residues), 132.3 bits, E=1.7e-42
• PF13537: GATase_7
  • amino acids 84 to 203 (120 residues), 144.1 bits, E=3.2e-46
• PF00733: Asn_synthase
  • amino acids 284 to 623 (340 residues), 285.3 bits, E=1.6e-88

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 97% identity to psa:PST_1440)

MetaCyc: 54% identical to N-acetylglutaminylglutamine amide synthase (Sinorhizobium meliloti 1021)
RXN-13456

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• N-acetylglutaminylglutamine amide biosynthesis (1/2 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.4

Sequence Analysis Tools

See L0GKZ5 at UniProt or InterPro

Protein Sequence (627 amino acids)

>Psest_2930 asparagine synthase (glutamine-hydrolyzing) (Pseudomonas stutzeri RCH2)
MTEPSQAGSTPLRWSEKLNFARDGSVTLPNGKTGKDLMCGIAGELRFDNRPADLAAVERI
TQHLTARGPDACGFHSQGPLALGHRRLKIMDLCEASGQPMIDSALGLSMVFNGAIYNYPE
LRAELEGLGYRFFSEGDTEVLLKGFHAWGEALLPRLNGMFAFAIWQRDTQQLFIARDRLG
VKPLYLSRTDQRLRFASSLPALLKGGDIAGVLNPVALNHYMSFHAVVPAPDTLIAGIEKL
PPASWMRVDANGATTTQRWWELQFGAREEERNYSFEDWKQRTLDTMREAVAIRQRAAVDV
GVLLSGGVDSSLLVGLLREAGVADNLLTFSIGFEDAGGERGDEFKYSDLIAKHYKTRHHQ
LRIQEKEILEQLPAAFQAMSEPMVSHDCIAFYLLSREVAKHCKVVQSGQGADELFAGYHW
YPLVDGAEDPVAAYLAAFRDRSYEEYAETVQQQWVQGDFSGDFVRQHFAQPGADAAVDKA
LRIDSTVMLVDDPVKRVDNMTMAWGLEARTPFLDYRVAELSARIPAKFKLPEGGKYVLKE
AARQVIPAEVIDRPKGYFPVPGLKHLQGATLNWVREMLLDPSQERGLYNPQALEKLLADP
DGQLTPLRGSKLWQLAAVNLWLSEQGL