Protein Info for Psest_2914 in Pseudomonas stutzeri RCH2

Annotation: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 776 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 31 to 48 (18 residues), see Phobius details amino acids 55 to 71 (17 residues), see Phobius details amino acids 80 to 100 (21 residues), see Phobius details amino acids 112 to 135 (24 residues), see Phobius details amino acids 698 to 714 (17 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 5 to 775 (771 residues), 1108.6 bits, see alignment E=0 PF13360: PQQ_2" amino acids 214 to 371 (158 residues), 36.1 bits, see alignment E=8.8e-13 PF01011: PQQ" amino acids 223 to 250 (28 residues), 27.1 bits, see alignment (E = 3.9e-10) amino acids 706 to 740 (35 residues), 26.2 bits, see alignment (E = 7.5e-10)

Best Hits

Swiss-Prot: 48% identical to DHG_ECOLI: Quinoprotein glucose dehydrogenase (gcd) from Escherichia coli (strain K12)

KEGG orthology group: K00117, quinoprotein glucose dehydrogenase [EC: 1.1.5.2] (inferred from 94% identity to psa:PST_1455)

MetaCyc: 48% identical to quinoprotein glucose dehydrogenase (Escherichia coli K-12 substr. MG1655)
Quinoprotein glucose dehydrogenase. [EC: 1.1.5.2]

Predicted SEED Role

"Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2)" in subsystem D-gluconate and ketogluconates metabolism or Entner-Doudoroff Pathway or Respiratory dehydrogenases 1 (EC 1.1.5.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNN7 at UniProt or InterPro

Protein Sequence (776 amino acids)

>Psest_2914 membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family (Pseudomonas stutzeri RCH2)
MAVVISGLFCIIVGIALGVGGAKVVSLGGTWYFAIVAAGFLLTGVLLLMRRRTALWVYAL
LMLGALGWAVYEVGLDWWQLAPRGSIIAPFGLWLLTPWIARRLGWQHFGFRAWGGAALPL
MLAVGLWGAATVYAVGHDGHDIKGMLPAPLAQAPAADDSVPAGDWHAYGRSQHGQRYSPL
AQITPQNVDRLEPVWHYQTGDLRGPDDPDETTYEVTPLKVDDSLYLCTPHNLVIALDAET
GQERWRFDPKVPHSVNRQHLTCRGLSYHAAPQGSDVQACRQRLFMPTADARLIALDAKTG
EICPGFADNGEIDLWANMPHAKEGFYYSTSPPVVARDLVIIGGAVNDNVRAQEPSGVIRA
YDVYTGQLKWNWDPGNPDATEPIAAGQTYSPSSPNSWSISSADEALGMVYVPLGNQVPDQ
WGGRRNENSERFSSSIVALDLDSGQLRWVFQTVRHDLWDMDVPAQPSLVDIQTENGPVPA
VVAPTKQGDIYVLDRRTGEPILPVREVPAPQGAAEGDWVAKTQPASALSYEPPKLQGKDL
WGATLIDQMMCHIQFHSLRYEGRYTPPSTQGTLVHPGNFGVFNWGGVAVDPVRQMVFSTP
AYLAFTSKLIPREDAETTYVSEQEPFLNENFGSPFAVELKAFVSPIGLPCTAPPWGYVAG
ADLRTGKTHWLRKNGTVRDRSPIPLPFKMGVPNLGGPMLTAGGVAFLSGTLDYYLRAYDV
MTGRELWKARLPAGGQATPMTYQSRSGRQMVVVVAGGHGSLGTKAGDSVIAYALPR