Protein Info for Psest_0286 in Pseudomonas stutzeri RCH2

Annotation: pilus retraction protein PilT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 381 transmembrane" amino acids 125 to 145 (21 residues), see Phobius details TIGR01420: twitching motility protein" amino acids 3 to 343 (341 residues), 453.4 bits, see alignment E=2.7e-140 PF00437: T2SSE" amino acids 67 to 278 (212 residues), 137.2 bits, see alignment E=2.8e-44

Best Hits

Swiss-Prot: 41% identical to PILT_PSEAE: Twitching mobility protein (pilT) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02670, twitching motility protein PilU (inferred from 92% identity to pfv:Psefu_0309)

Predicted SEED Role

"Twitching motility protein PilT" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGL1 at UniProt or InterPro

Protein Sequence (381 amino acids)

>Psest_0286 pilus retraction protein PilT (Pseudomonas stutzeri RCH2)
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQR
REFTENHECNFAISARGIGRFRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSM
TKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIEDPIEFIHQHQSCIVTQREVGI
DTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVH
ELKSLMKRSTELGMQTFDQALYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHL
TSVSQGLSLEVSDDDPGRSFR