Protein Info for Psest_2883 in Pseudomonas stutzeri RCH2

Annotation: Predicted membrane protein involved in D-alanine export

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 512 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 31 to 47 (17 residues), see Phobius details amino acids 53 to 69 (17 residues), see Phobius details amino acids 76 to 95 (20 residues), see Phobius details amino acids 115 to 133 (19 residues), see Phobius details amino acids 145 to 165 (21 residues), see Phobius details amino acids 185 to 203 (19 residues), see Phobius details amino acids 246 to 266 (21 residues), see Phobius details amino acids 282 to 300 (19 residues), see Phobius details amino acids 321 to 342 (22 residues), see Phobius details amino acids 348 to 369 (22 residues), see Phobius details amino acids 381 to 403 (23 residues), see Phobius details amino acids 409 to 426 (18 residues), see Phobius details amino acids 433 to 452 (20 residues), see Phobius details amino acids 482 to 503 (22 residues), see Phobius details PF03062: MBOAT" amino acids 141 to 300 (160 residues), 65.5 bits, see alignment E=2.8e-22

Best Hits

KEGG orthology group: None (inferred from 92% identity to psa:PST_2877)

Predicted SEED Role

"Probable poly(beta-D-mannuronate) O-acetylase (EC 2.3.1.-)" in subsystem Alginate metabolism (EC 2.3.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNK9 at UniProt or InterPro

Protein Sequence (512 amino acids)

>Psest_2883 Predicted membrane protein involved in D-alanine export (Pseudomonas stutzeri RCH2)
MLFNSVEFIAGFLPVVLIGFFVLTGSGQQRLAVIWLTVVSLVFYGWWNPVYVPLLVGSML
VNYLLGGYLRRHPSRLLLGLAVAANVLLLVYYKYTGFLLGTLDAALDLGWRVEDIILPLA
ISFFTFQQIAYLVDAHDGVVEEHDFTNYCLFISFFPQLIAGPITHHGEMLAQFRDRDSFR
ARIDNLSLGATVFLLGLFKKVVIADTLALKATPVFSLAADGTIPAFYDAWTGALTYTLQI
YFDFSGYSDMAIGLALLFGISLPANFNSPFKARNVIDYWSRWHMTLTRFLTAYLYNPIVL
RVTRARMAAGKPQPRRGKMTVGTFFALVAYPTVFTMFISGIWHGAGWHFVAFGLLHGVYL
VVAHGWRAWKARQGWKLDSDIWWHKAAAVSLTFGCVVVAMVFFRASDVPTAMAILAGMLG
LSPISTAMDRSDFVYIAALLVFVWGMPNVQQWMGHFRTALNFQAQPHWTERLLPVSAWRP
TAIHGLAVGVLGFFALAIAFSMAPTEFLYFQF