Protein Info for Psest_2851 in Pseudomonas stutzeri RCH2

Annotation: NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 930 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 77 to 97 (21 residues), see Phobius details amino acids 108 to 125 (18 residues), see Phobius details amino acids 131 to 151 (21 residues), see Phobius details amino acids 163 to 185 (23 residues), see Phobius details amino acids 204 to 224 (21 residues), see Phobius details amino acids 241 to 263 (23 residues), see Phobius details amino acids 269 to 291 (23 residues), see Phobius details amino acids 298 to 316 (19 residues), see Phobius details amino acids 322 to 346 (25 residues), see Phobius details amino acids 367 to 386 (20 residues), see Phobius details amino acids 406 to 427 (22 residues), see Phobius details amino acids 460 to 483 (24 residues), see Phobius details amino acids 502 to 522 (21 residues), see Phobius details amino acids 569 to 589 (21 residues), see Phobius details amino acids 601 to 621 (21 residues), see Phobius details amino acids 626 to 646 (21 residues), see Phobius details amino acids 652 to 674 (23 residues), see Phobius details amino acids 686 to 708 (23 residues), see Phobius details amino acids 749 to 766 (18 residues), see Phobius details amino acids 788 to 809 (22 residues), see Phobius details amino acids 816 to 836 (21 residues), see Phobius details amino acids 848 to 872 (25 residues), see Phobius details amino acids 894 to 915 (22 residues), see Phobius details PF00662: Proton_antipo_N" amino acids 65 to 110 (46 residues), 34.3 bits, see alignment 4.4e-12 PF00361: Proton_antipo_M" amino acids 126 to 401 (276 residues), 225.7 bits, see alignment E=1.8e-70 PF13244: MbhD" amino acids 611 to 674 (64 residues), 66 bits, see alignment 7e-22 PF20501: MbhE" amino acids 685 to 767 (83 residues), 98.5 bits, see alignment E=4.8e-32 PF04039: MnhB" amino acids 789 to 911 (123 residues), 131.6 bits, see alignment E=5e-42

Best Hits

KEGG orthology group: K05559, multicomponent K+:H+ antiporter subunit A (inferred from 96% identity to psa:PST_1529)

Predicted SEED Role

"Na(+) H(+) antiporter subunit A; Na(+) H(+) antiporter subunit B"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPP8 at UniProt or InterPro

Protein Sequence (930 amino acids)

>Psest_2851 NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit (Pseudomonas stutzeri RCH2)
MALALIIALPFLGIFLPLFLERFGRSACAFGAGLAPLAALILLLSKRADVFAGQTEIIHY
DWLPQLGLNLSLRLDGLGFLFALLILGIGLLVILYARYYLSKKDPMGRFYGFFLLFMGAM
LGVVLSENLLLMLMFWELTSLSSFLLIGYWNYRADARQGARMALAVTGGGGLALLAGILL
IGYIVGSFELTTVLASGDLIRANALYPVALILVLLGVFTKSAQFPFHFWLPQAMAAPTPV
SAFLHSATMVKAGVFLLARLYPALAESEWWFYLVSITGLTTLLLGAVMALFQHDLKGLLA
YSTISHLGLITLLFGLDSRLAAVAAIFHIINHATFKASLFMAAGIIDHETGTRDMRRING
MWKYMPHTAALAMVASLAMAGVPLLNGFLSKEMFFAETLDQHLLGSFYWTIPAAATLAGA
FSVAYSLRFVHDVFFNGEPIDLPKYPPHEPARYMKIPVEILVLLCLLVGMLPAFTVGPLL
AAAVSGTLNGNVPEYSLSIWHGFNLPLAMSFVALIGGILIYSLRKWAFRWYEGLPTVDSL
EVFERVMAGLTLGARYITSLLENGSLQRYIALMLISTLVLVVFALTPLQTLHGTVALTPL
DGITVAGMVILALTSLLTVLFHRRRLVALMVLSVVGLMVALGFARYSAPDLALTQLSVEV
VTIILLILALFFMPDRTPAESSSLRAFRDFILAGGCGTMVALLAYTVLTRPYDSIASFFL
ENSVSGGGGTNVVNVILVDFRGFDTLGEIAVLAIAGVGIYSLLYGLRLPHPIRDAGGRLW
SLDKHPMMLDALARALLPMALLISAFIFLRGHNLPGGGFIAGLITAVALILQYIAHGVAW
AQARQPFSYHAMCGAGLLIAGATGLGSLLFGHSFLTSAFDHFHLPLIGEFELATAMLFDL
GVYLVVVGATLLILSNIGQVRQDETSREVL