Protein Info for Psest_2845 in Pseudomonas stutzeri RCH2

Annotation: [Protein-PII] uridylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 TIGR01693: protein-P-II uridylyltransferase" amino acids 36 to 885 (850 residues), 1106.9 bits, see alignment E=0 PF01909: NTP_transf_2" amino acids 65 to 111 (47 residues), 35.5 bits, see alignment 2.2e-12 PF08335: GlnD_UR_UTase" amino acids 185 to 321 (137 residues), 158.1 bits, see alignment E=2.9e-50 PF01966: HD" amino acids 462 to 560 (99 residues), 41.7 bits, see alignment E=2.6e-14 PF01842: ACT" amino acids 815 to 865 (51 residues), 34.8 bits, see alignment 2.2e-12

Best Hits

Swiss-Prot: 98% identical to GLND_PSEU5: Bifunctional uridylyltransferase/uridylyl-removing enzyme (glnD) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 98% identity to psa:PST_1535)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPU9 at UniProt or InterPro

Protein Sequence (900 amino acids)

>Psest_2845 [Protein-PII] uridylyltransferase (Pseudomonas stutzeri RCH2)
MPQVDPELFDRSQFQAELALKASPIAAYKKAIRQARQVLDERFKAGRDIRRLIQDRAWFV
DQILRSAWDRFDWNKGADIALVAVGGYGRGELHPYSDIDLLILLDENDQEVFRNSIEGFL
TLLWDIGLEVGQAVRSVAECAEEARADLTVITNLMESRTIAGPERLRQAMLQVTRTEQMW
PSKEFFLAKRNEQRARHAKYNNTEYNLEPNVKGSPGGLRDIQTILWIARREFGTLNLQAM
VDQGFLTEGEHSLLTAAQEFLWKVRYGLHMLAGRAEDRLLFDHQRSLAALLGYEDNDAKL
AIERFMQKYYRVVMSIAELSDLVGQHFAEVILWEGESGPIVPLNSRFQVRDGYLEVSNPA
IFKRTPFAILETFVLLAQHPDIQGVRSDTIRLLRDHRYLIDDVFRQDLRNTSLFIELFKC
KEGIHRNLRRMNRYGILGRYLPEFGHIVGQMQHDLFHIYTVDAHTLNVIKYLRKLTKPGV
AEKYPLASKLVEKLPKPELIYIAGLYHDIAKGRGGDHSELGAVDAEQFCSRHKLPAWDTR
LVVWLVENHLVMSTTAQRKDLSDPQVINDFAQLVGDETHLDYLYVLTVADINATNPTLWN
SWRASLLRQLYTETKRALKRGLENPLGREEQIRQTQRAALDDLVRHGTDPDDAEQLWAQL
GDDYFLRHTATDVAWHTDAIIEHPANGGPLVLIKETTQREFEGGTQIFIYAPDQHDFFAV
TVAAMDQLNLNIHDARILTSSSQFTLDTYIVLDADGSPIGNNPERIDEIRKGLIAALRNP
DDYLTIIQRRVPRQLKHFAFPPQVTIHNDTQRPQTILEIIAPDRPGLLARVGQLFLDFDL
SVQNAKIATLGERVEDVFFVTDADNQPLSDPQFCLRLQQALIKELQQENEQQPSPSSIVI