Protein Info for Psest_2825 in Pseudomonas stutzeri RCH2

Annotation: tRNA(Ile)-lysidine synthetase, N-terminal domain/tRNA(Ile)-lysidine synthetase, C-terminal domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 429 transmembrane" amino acids 20 to 40 (21 residues), see Phobius details PF01171: ATP_bind_3" amino acids 20 to 195 (176 residues), 187.3 bits, see alignment E=3.5e-59 TIGR02432: tRNA(Ile)-lysidine synthetase" amino acids 21 to 200 (180 residues), 168.8 bits, see alignment E=1.3e-53 PF09179: TilS" amino acids 252 to 317 (66 residues), 50 bits, see alignment E=4.3e-17 PF11734: TilS_C" amino acids 359 to 413 (55 residues), 60.7 bits, see alignment 1e-20 TIGR02433: tRNA(Ile)-lysidine synthetase, C-terminal domain" amino acids 359 to 403 (45 residues), 50.4 bits, see alignment 1.1e-17

Best Hits

Swiss-Prot: 61% identical to TILS_PSESM: tRNA(Ile)-lysidine synthase (tilS) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K04075, tRNA(Ile)-lysidine synthase [EC: 6.3.4.-] (inferred from 70% identity to psa:PST_1554)

Predicted SEED Role

"tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)" (EC 6.3.4.19)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.- or 6.3.4.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPM3 at UniProt or InterPro

Protein Sequence (429 amino acids)

>Psest_2825 tRNA(Ile)-lysidine synthetase, N-terminal domain/tRNA(Ile)-lysidine synthetase, C-terminal domain (Pseudomonas stutzeri RCH2)
MSLDSILLSALAPWRSSPTWWVAFSGGLDSTVLLHLLARLADRHELPVIRAIHVHHGLQV
IADDWPAHCQRICDELGVALSVIQVQVGSGASVEQAARNARYQAFSQCLSEDELLLTGQH
RDDQAETVLFRLLRGAGVRGLAAMPGERPLGHGRLVRPLLGVLRDELEAYAERNALTWVE
DPSNQQYAHTRNYLRWEILPLLRQRWPGAISALARTATHMAEAQQLLDELAQQDLAAAKA
ADSLGWLTLPSLALPALRAVTPVRQRNALRYWLAGLTLAPDEAHWVGWISVRDAKPDATP
CWRLVGGELQRCGDRIWWVPEAWLEPPVAPVDWPDSTARLALPGNGTLRMEGLAPPGKLQ
VRYRQGGEAMLVAGRGRRDLKRLLNEAGVPVFVRARLPLLYCDEELVAVANLPQLSKGGV
ALDWCVPEF