Protein Info for Psest_2806 in Pseudomonas stutzeri RCH2

Annotation: RNA polymerase sigma factor RpoS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 331 TIGR02394: RNA polymerase sigma factor RpoS" amino acids 53 to 330 (278 residues), 461.1 bits, see alignment E=1.5e-142 PF00140: Sigma70_r1_2" amino acids 58 to 91 (34 residues), 50.3 bits, see alignment 3.7e-17 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 93 to 319 (227 residues), 122.4 bits, see alignment E=1.4e-39 PF04542: Sigma70_r2" amino acids 96 to 165 (70 residues), 83.1 bits, see alignment E=2e-27 PF04539: Sigma70_r3" amino acids 176 to 250 (75 residues), 74.6 bits, see alignment E=1.1e-24 PF04545: Sigma70_r4" amino acids 264 to 317 (54 residues), 56.8 bits, see alignment 2.5e-19

Best Hits

Swiss-Prot: 87% identical to RPOS_PSEAE: RNA polymerase sigma factor RpoS (rpoS) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03087, RNA polymerase nonessential primary-like sigma factor (inferred from 98% identity to psa:PST_1572)

MetaCyc: 75% identical to RNA polymerase sigma factor RpoS (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"RNA polymerase sigma factor RpoS" in subsystem Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMS9 at UniProt or InterPro

Protein Sequence (331 amino acids)

>Psest_2806 RNA polymerase sigma factor RpoS (Pseudomonas stutzeri RCH2)
MALKDQALEFDVDDAVLLMETPIHLDQKSNVTRAATAGRPKAASSKQHKFIDYNRALDAT
QLYLNEIGFSPLLTPEEEVHFARLAQKGDPAGRKRMIESNLRLVVKIARRYVNRGLSLLD
LVEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKELNVY
LRAARELTQKLDHEPSPEEIANLLEKPVGEVKRMLGLNERVTSVDVSLGPDTDKTLLDTL
TDDRPTDPCELLLDDDLSNSIDQWLSDLSEKQREVVIRRFGLRGHEASTLEEVGQEIGLT
RERVRQIQVEALRRLREILERNGLSSDALFQ