Protein Info for Psest_2719 in Pseudomonas stutzeri RCH2

Annotation: acetylornithine and succinylornithine aminotransferases/succinylornithine transaminase family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03246: succinylornithine transaminase family
  • amino acids 8 to 403 (396 residues), 708.1 bits, E=3.2e-217
• TIGR00707: transaminase, acetylornithine/succinylornithine family
  • amino acids 17 to 400 (384 residues), 506.7 bits, E=3.6e-156
• PF00202: Aminotran_3
  • amino acids 26 to 400 (375 residues), 429.3 bits, E=1.3e-132
• PF00155: Aminotran_1_2
  • amino acids 173 to 329 (157 residues), 20.6 bits, E=2.3e-08

Best Hits

Swiss-Prot: 80% identical to ARUC_PSEAE: Succinylornithine transaminase/acetylornithine aminotransferase (aruC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00821, acetylornithine/N-succinyldiaminopimelate aminotransferase [EC: 2.6.1.11 2.6.1.17] (inferred from 96% identity to psa:PST_1649)

MetaCyc: 80% identical to succinylornithine transaminase subunit (Pseudomonas aeruginosa)
Succinylornithine transaminase. [EC: 2.6.1.81]; Ornithine aminotransferase. [EC: 2.6.1.81, 2.6.1.13]; Acetylornithine transaminase. [EC: 2.6.1.81, 2.6.1.13, 2.6.1.11]

Predicted SEED Role

"Succinylornithine transaminase (EC 2.6.1.81)" in subsystem Arginine and Ornithine Degradation (EC 2.6.1.81)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-arginine degradation XIII (reductive Stickland reaction) (5/5 steps found)
• L-ornithine biosynthesis I (5/5 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-ornithine biosynthesis II (3/3 steps found)
• L-proline biosynthesis III (from L-ornithine) (3/3 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-arginine degradation VI (arginase 2 pathway) (3/4 steps found)
• L-N^δ-acetylornithine biosynthesis (5/7 steps found)
• L-arginine degradation I (arginase pathway) (2/3 steps found)
• L-arginine degradation II (AST pathway) (2/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.17

Use Curated BLAST to search for 2.6.1.11 or 2.6.1.13 or 2.6.1.17 or 2.6.1.81

Sequence Analysis Tools

See L0GN53 at UniProt or InterPro

Protein Sequence (406 amino acids)

>Psest_2719 acetylornithine and succinylornithine aminotransferases/succinylornithine transaminase family (Pseudomonas stutzeri RCH2)
MSAPHTPVERADFDQVIVPTFAPAAFVPVRGLGSRVWDQSGRELVDFAGGIAVNALGHAH
PAMVAALTEQAGKLWHISNIYTNEPALRLAKKLVAATFADRAFFCNSGAEANEAAFKLAR
RYAHDVYGPQKFEIISALNSFHGRTLFTVTVGGQSKYSDGFGPKIEGITHVPYNDLEALK
AAISDKTCAVVLEPIQGESGILPGEQAYLEGARQLCNEHNALLIFDEVQTGMGRTGELFA
YMHYGITPDILTNAKSLGGGFPIGAMLTTNEIAAHLSVGTHGTTYGGNPLACAVAEAVVD
IVNTPEVLEGVKAKHERFKARLTQIGERYGVFSLVRGRGLLIGCVLSDAWKGKAGAFCAA
AEKEALMVLQAGPDVVRLAPSLVIDQADIDEGLDRLERAVAALTQA