Protein Info for Psest_2706 in Pseudomonas stutzeri RCH2

Annotation: tRNA dihydrouridine synthase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 TIGR00742: tRNA dihydrouridine synthase A" amino acids 16 to 331 (316 residues), 467.1 bits, see alignment E=1.6e-144 PF01207: Dus" amino acids 20 to 325 (306 residues), 300.9 bits, see alignment E=5e-94

Best Hits

Swiss-Prot: 84% identical to DUSA_PSEPK: tRNA-dihydrouridine(20/20a) synthase (dusA) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K05539, tRNA-dihydrouridine synthase A [EC: 1.-.-.-] (inferred from 90% identity to psa:PST_1661)

MetaCyc: 62% identical to tRNA-dihydrouridine synthase A (Escherichia coli K-12 substr. MG1655)
1.1.1.-

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPG9 at UniProt or InterPro

Protein Sequence (334 amino acids)

>Psest_2706 tRNA dihydrouridine synthase A (Pseudomonas stutzeri RCH2)
MYESAIAETTRPAPSRRFSVAPMMDWTDRHCRYFLRQLSRHALLYTEMVTTGALLHGDAQ
RFLRHDESEHPLALQLGGSVPAELAACAKLAEAAGYDEVNLNVGCPSDRVQNNMIGACLM
GHPQLVADCVKAMRDAVGVEVTVKHRIGINGRDSYAELCDFVGQVRDAGCRSFTVHARIA
ILEGLSPKQNREVPPLRYDIAAQLKQDFPDLEIILNGGIKTLDECRAHLQTFDGVMLGRE
AYHNPYLLARVDHELFGSDTPVIGRAQALRNMRPYIEQHLAEGGAMHHITRHVLGLGQGF
PGARRFRQLLSVDIHKTSQPLALLDQAVTLLEGH