Protein Info for Psest_0263 in Pseudomonas stutzeri RCH2

Annotation: PAS domain S-box/diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 642 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 38 to 59 (22 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 78 to 201 (124 residues), 69.3 bits, see alignment E=3.3e-23 PF13188: PAS_8" amino acids 84 to 123 (40 residues), 34.2 bits, see alignment 5.8e-12 PF00989: PAS" amino acids 84 to 190 (107 residues), 56.4 bits, see alignment E=1e-18 PF08448: PAS_4" amino acids 86 to 195 (110 residues), 35.7 bits, see alignment E=3.1e-12 PF13426: PAS_9" amino acids 91 to 192 (102 residues), 55.9 bits, see alignment E=1.6e-18 PF08447: PAS_3" amino acids 102 to 187 (86 residues), 42.3 bits, see alignment E=2.6e-14 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 201 to 365 (165 residues), 123.5 bits, see alignment E=7.2e-40 PF00990: GGDEF" amino acids 205 to 362 (158 residues), 145.6 bits, see alignment E=4.2e-46 PF00563: EAL" amino acids 384 to 618 (235 residues), 266.1 bits, see alignment E=9e-83

Best Hits

KEGG orthology group: None (inferred from 90% identity to psa:PST_3987)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHT4 at UniProt or InterPro

Protein Sequence (642 amino acids)

>Psest_0263 PAS domain S-box/diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MNTYLVRLSLGYFSLAMLWILLGDTLVNHLAAGDSQAWQMGKGALFVALTSALIFLLGRQ
HFRRFDEQQRAQHVQETSLKQAAAVFDSTQEGVLVTDPRQRIVHVNPAFSRITGYSTEEV
LGQTPKLFASGKHDAEFYQQMWLALKDKGEWSGEIWNRRKNGEVYPQWQNLRCIHDNQGQ
LSHYVAVFSDLSALKRSREELDQLAHYDPLVNLPNRLLFTERAKQDLERARAHKRSGALL
LIDLDHFKDINESLGHSLGDALLQAVSTRLSEHLEKGMTLGRLGGDEFALLCEGYGAEKA
TALALRILDRLNESFHIGDQELFSSASIGIVLYPYPTDVQNAEQLMRNADSALFKAKSSG
RSTYAFYSEELTSQARQRVELVTALRQALEQGQLRLHYQPIYDLRQGTISGFEALVRWQH
PEKGLIAPGVFIPIAEETGLISAIDHWVLEQACNQAREWLGQGHPLGFIAVNISSRLFGN
GELDLQVATILARSGLEARHLELEITESAVMQDPDAALELLQRLRALGVQLAIDDFGTGY
SSLQRLKRMNVHKLKIDQGFVRGLPDDRDDAAITRSVIGLAHNLGLKVVAEGIETAEQAA
FLLAQNCDYGQGYGFARPQPADAIDWSPSALCHGQACRSSGR