Protein Info for Psest_2664 in Pseudomonas stutzeri RCH2

Annotation: tripartite ATP-independent periplasmic transporter solute receptor, DctP family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR00787: TRAP transporter solute receptor, DctP family" amino acids 30 to 280 (251 residues), 256.9 bits, see alignment E=9.9e-81 PF03480: DctP" amino acids 30 to 312 (283 residues), 374.4 bits, see alignment E=1.9e-116

Best Hits

Swiss-Prot: 77% identical to DCTP_PSEAE: C4-dicarboxylate-binding periplasmic protein DctP (dctP) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 90% identity to psa:PST_1699)

Predicted SEED Role

"TRAP-type C4-dicarboxylate transport system, periplasmic component"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPD3 at UniProt or InterPro

Protein Sequence (329 amino acids)

>Psest_2664 tripartite ATP-independent periplasmic transporter solute receptor, DctP family (Pseudomonas stutzeri RCH2)
MLSNKLKALACALSFSIAGLVHADPVTIKFAHVVADNTPKGQGALLFKKLAEERLPGKVK
VEVYPNSSLFGDGKEMEALLLGDVHMLAPSLAKFEHYAKAIQIYDLPFLFDDLAAADRFQ
SGPQGKALLRAMEDKNITGLAYWHNGMKQLSANKPLREPKDARGLKFRVQASAVLDEQFK
AVRANPRKMSFAEVYQGLQTGVVNGTENTWSNYESQKVHEVQPYMTASDHGLIDYMVITN
TKFWNGLPEDLRGELETIMEEVTAEVNRQADDLNQQARQAIAASGKTEIIELTPEQRAQW
REAMKPVWKKFENEIGAELIEAAQAANRS