Protein Info for PGA1_c26430 in Phaeobacter inhibens DSM 17395

Annotation: glutamyl-tRNA synthetase GltX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00749: tRNA-synt_1c
  • amino acids 3 to 305 (303 residues), 249.3 bits, E=4.6e-78
• TIGR00464: glutamate--tRNA ligase
  • amino acids 3 to 435 (433 residues), 333.9 bits, E=9e-104

Best Hits

Swiss-Prot: 88% identical to SYE2_RUEST: Glutamate--tRNA ligase 2 (gltX2) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 88% identity to sit:TM1040_2275)

Predicted SEED Role

"Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.24)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (28/33 steps found)
• tRNA charging (19/21 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• superpathway of heme b biosynthesis from glutamate (7/10 steps found)
• tetrapyrrole biosynthesis I (from glutamate) (4/6 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (24/36 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism
• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.17

Use Curated BLAST to search for 6.1.1.17 or 6.1.1.24

Sequence Analysis Tools

See I7DTC0 at UniProt or InterPro

Protein Sequence (441 amino acids)

>PGA1_c26430 glutamyl-tRNA synthetase GltX (Phaeobacter inhibens DSM 17395)
MTTTRFAPSPTGYIHVGNLRTALMNYLIARKAGGSFILRIDDTDPERSKEEYVDAIKQDL
EWLGLGWDRVERQSERLERYVAAADKLREIGRFYEAFETPVELDLKRKKQLNMGKPPVYD
RAALALSDDEKAALRAERGDGVWRFKLDQQRIEWADGILGDISIDAASVSDPVLIRGDGQ
FLYTLASVVDDTEMGVTHVVRGSDHVTNTATQIQIIEALGGTVPSFAHHSLLTGPQGEAL
SKRLGTLALRDLREAGVQPMALLSMMARLGSSDPVEIRSEMAELIDGFDINRFGAAPTKF
DVEDLYPLTARYLQSLSLADVQTHVDALGVPAEKQAAFWDVAKENISTLKDLGGWWELCR
DGAEPLIAEEDTAFIAEAMALLPEGPYDGESWGKWTAAVKEKTGRKGKGLFMPLRKAVTG
MERGPDMSALLALMETVRARG