Protein Info for Psest_2633 in Pseudomonas stutzeri RCH2

Annotation: choline dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 557 PF00732: GMC_oxred_N" amino acids 3 to 299 (297 residues), 196.2 bits, see alignment E=6.6e-62 TIGR01810: choline dehydrogenase" amino acids 4 to 536 (533 residues), 921.2 bits, see alignment E=1e-281 PF05199: GMC_oxred_C" amino acids 392 to 523 (132 residues), 137.8 bits, see alignment E=3.7e-44

Best Hits

Swiss-Prot: 83% identical to BETA_PSEAB: Oxygen-dependent choline dehydrogenase (betA) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K00108, choline dehydrogenase [EC: 1.1.99.1] (inferred from 93% identity to psa:PST_1746)

MetaCyc: 83% identical to choline dehydrogenase (Pseudomonas aeruginosa)
Choline dehydrogenase. [EC: 1.1.99.1]

Predicted SEED Role

"Choline dehydrogenase (EC 1.1.99.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.1.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPA4 at UniProt or InterPro

Protein Sequence (557 amino acids)

>Psest_2633 choline dehydrogenase (Pseudomonas stutzeri RCH2)
MEYDYIIIGAGSAGNVLAARLTEDADVSVLLLEAGGPDYRLDFRTQMPAALAYPLQGTRY
NWAYKTDPEPHMNNRRMDCGRGKGLGGSSLINGMCYIRGNALDYDNWAKAPGLEDWTYLD
CLPYFRKAESRDIGPNDYHGGVGPVSVTTPRADNNVLFQAMVEAGVQAGYPRTDDLNGYQ
QEGFGPMDRTVTPQGRRASTARGYLDQARERPNLTIETHAVTDRVLFEGKRAVGVRYLRD
RKDPYIARARREVLLCGGAIASPQILQRSGVGPGALLRRVGVKPVHELPGVGQNLQDHLE
MYLQYECKQPVSLYPALKWWNQPAIGAEWLFLGSGIGASNQFEAGGFIRSSDEFEWPNIQ
FHFLPVAVSYNGSNAHDGHSFQAHVGSMRSPSRGRIEIRSTDPAEDPSILFNYMAHEQDW
REFRDGIRLTREIMNQPALDPYRGRELSPGIEAQSDAELDAFVREHAETAYHPSCSCKMG
TDDMAVVDGQGRVHGMEGLRVVDASIMPQIITGNLNATTIMIAEKIADRIRGREPLRRST
AKYYVAGNVPVRQPPRR