Protein Info for Psest_0253 in Pseudomonas stutzeri RCH2

Annotation: Predicted hydrolase of the alpha/beta-hydrolase fold

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 PF12146: Hydrolase_4" amino acids 58 to 171 (114 residues), 38.4 bits, see alignment E=1.3e-13 PF00561: Abhydrolase_1" amino acids 58 to 306 (249 residues), 85.7 bits, see alignment E=6.4e-28 PF12697: Abhydrolase_6" amino acids 60 to 305 (246 residues), 49.2 bits, see alignment E=1.7e-16

Best Hits

KEGG orthology group: K07019, (no description) (inferred from 92% identity to psa:PST_3996)

Predicted SEED Role

"Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase" in subsystem cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHS5 at UniProt or InterPro

Protein Sequence (328 amino acids)

>Psest_0253 Predicted hydrolase of the alpha/beta-hydrolase fold (Pseudomonas stutzeri RCH2)
MPDTFQPAWWLPGAHLQTLWNPFFRKPPRLERQRERLWLADGDFIDLDWHGPHEATAPLV
LVLHGLTGSSNSLYVLGLQQQLAARGWASVAINWRGCSGEPNLLPRAYHSGASDDLAEVI
GHLQASRPLAPLYAVGYSLGGNVLLKYLGETAASSPLRKAVAVSVPFRLDQCADRIGLGF
SRIYQAHFMKAMVAYVKDKQQRFQREGLQEHLGALQSLGPLHGMRTFWDFDGRFTAPLHG
YSDAQDYYRRASSRYYLPTITTPTLLIQAEDDPFVFRHSVPDAAELSATTTLELHPRGGH
VGFIEGSPRRPRYYLEQRIPQWLAEATA