Protein Info for Psest_2562 in Pseudomonas stutzeri RCH2

Annotation: PAS domain S-box/diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 778 transmembrane" amino acids 30 to 49 (20 residues), see Phobius details PF13426: PAS_9" amino acids 115 to 209 (95 residues), 19.4 bits, see alignment E=3.7e-07 amino acids 233 to 337 (105 residues), 34.8 bits, see alignment E=5.8e-12 TIGR00229: PAS domain S-box protein" amino acids 115 to 210 (96 residues), 33.4 bits, see alignment E=4.2e-12 amino acids 219 to 345 (127 residues), 58.6 bits, see alignment E=6.9e-20 PF08447: PAS_3" amino acids 117 to 202 (86 residues), 58.5 bits, see alignment E=2.4e-19 amino acids 246 to 332 (87 residues), 27.8 bits, see alignment E=8.6e-10 PF13188: PAS_8" amino acids 223 to 271 (49 residues), 31 bits, see alignment 6.3e-11 PF00989: PAS" amino acids 223 to 335 (113 residues), 32.1 bits, see alignment E=3.5e-11 PF08448: PAS_4" amino acids 228 to 339 (112 residues), 24 bits, see alignment E=1.3e-08 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 345 to 508 (164 residues), 153.5 bits, see alignment E=4.3e-49 PF00990: GGDEF" amino acids 349 to 506 (158 residues), 163.1 bits, see alignment E=1.7e-51 PF00563: EAL" amino acids 527 to 760 (234 residues), 228.8 bits, see alignment E=2.2e-71

Best Hits

KEGG orthology group: None (inferred from 90% identity to psa:PST_1804)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GP35 at UniProt or InterPro

Protein Sequence (778 amino acids)

>Psest_2562 PAS domain S-box/diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MGPRRDRRVRRCGASAEPMAAANAQLHWRCGWSASCSVVAFCLFGSFVLRPELSFVSAGR
AATTASMPPMTLDSSANGPSTLLSCEVLDRSRVLDTLINNLEGMAYRCRNDASWTMIFVS
QGALGLCGYSAAELVDETRITWERITHADDRLRVRRCIEQAVAAVGRFCVHYRIRTASGA
HKWVIERGVAVADEQGEIVVEGFIEDITAQRAILDALEQAELRYRSIFENASEGIFQSTR
DGRYLAANPALARIYGYDSATELVADLADIERRLYVEGGRREAFCRLMEHQGEVLNFESE
VYRRDGTRIWISENAHVVRGANGEFICYEGTVQDISERKHYQQQLERQANHDLLTGLPNR
ILLNDRIEQGLARAARLGYYLTLVFIDLDNFKFINDGLGHVAGDELLKSIAARLAGSLRG
SDTVARVGGDEFVLVLSDHYRISTVISLLERVLNEIRRPVTLAGREFQVGASIGVAMFPG
DGEDAQTLLKHADIAMYAAKKRGRNNFQFFTHDLNRIADERLNLEAAMRAALERDEFSVH
YQPKVDASRRIVGVEALARWTDAQLGVIGPDRFIPVAEESGLIMPLTLAVLRRAFSDARQ
WNAGRAIPLMVAVNLSPLLFLGDDVVDRVCTVLDDVGLPPNQVELEITETVFLGDGTRAV
AILAEFKARGFRLAMDDFGTGYSALSYLRRFPLDIIKIDRSLVTGLEQEEEVAMIARAAI
SLGKSLHKTVVAEGVENAAQFDFLCRNGCDEFQGYLLSRPLPAAQFTALLAAGGIVRF