Protein Info for Psest_2554 in Pseudomonas stutzeri RCH2

Annotation: NADH:flavin oxidoreductases, Old Yellow Enzyme family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 676 PF00724: Oxidored_FMN" amino acids 6 to 328 (323 residues), 307.3 bits, see alignment E=6.2e-95 PF07992: Pyr_redox_2" amino acids 377 to 646 (270 residues), 85.3 bits, see alignment E=2.1e-27 PF00890: FAD_binding_2" amino acids 378 to 415 (38 residues), 22.8 bits, see alignment 2e-08 PF12831: FAD_oxidored" amino acids 378 to 415 (38 residues), 33.6 bits, see alignment 1.1e-11 PF13450: NAD_binding_8" amino acids 380 to 415 (36 residues), 33.2 bits, see alignment 2.1e-11

Best Hits

Swiss-Prot: 62% identical to FADH_ECOLI: 2,4-dienoyl-CoA reductase (fadH) from Escherichia coli (strain K12)

KEGG orthology group: K00219, 2,4-dienoyl-CoA reductase (NADPH2) [EC: 1.3.1.34] (inferred from 96% identity to psa:PST_1812)

MetaCyc: 62% identical to 2,4-dienoyl-CoA reductase (Escherichia coli K-12 substr. MG1655)
2,4-dienoyl-CoA reductase (NADPH). [EC: 1.3.1.34]

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.34

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GM65 at UniProt or InterPro

Protein Sequence (676 amino acids)

>Psest_2554 NADH:flavin oxidoreductases, Old Yellow Enzyme family (Pseudomonas stutzeri RCH2)
MTAFPKLLAPLDLGFTTLRNRTLMGSMHTGLEEMPNGFERMAAFFAERARGGVGLIVTGG
VGPNEEGSVRAGAAKLSTPEEAEEHNIVTQAVHEAGGKICMQILHAGRYAYSPQLVAPSA
IQAPINPFTPRELDEEGIEKQIRDFVNCASLARQAGYDGVEIMGSEGYFINQFLVAHTNH
RTDRWGGSYENRMRLPVEIVRRVREAVGPDFIIIYRLSMLDLIEGGSIWEEVVQLAKAIE
QAGATLINTGIGWHEARIPTIATKVPRAAFTKVTAKLRGEVSIPLITTNRINTPEVAEQV
LAEGDADMVSMARPFLADPDFVNKAAAGHSERINTCIGCNQACLDHTFSGKLTTCLVNPR
ACHETELNYIPTTTVKKIAVVGAGPAGLAAATIAAERGHQVTLIDSAGEIGGQFNVAKRV
PGKEEFYETLRYFQNRLQETGVEVQLNTRATVETLLAGGFDDIILATGIAPRIPAIPGID
HPKVIGYLDAILERKPVGQQVAVIGAGGIGFDVSEYITHDGESTSLDREAFWKEWGIDLG
LAVRGGIAGIQPAPHAPKRQVYLLQRKKSKVGNGLGKTTGWIHRTGLKNKHVQMLNSVEY
LQIDDAGLHIRVGEGEPQVLPVDTVILCAGQEPLRELQEGLEAAGARVHLIGGADVAAEL
DAKRAINQGCRLAAAL