Protein Info for Psest_2543 in Pseudomonas stutzeri RCH2

Annotation: ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 248 PF00005: ABC_tran" amino acids 19 to 157 (139 residues), 112.9 bits, see alignment E=1.9e-36

Best Hits

Swiss-Prot: 46% identical to Y3647_BRUA2: Putative ATP-binding protein BAB2_1147 (BAB2_1147) from Brucella abortus (strain 2308)

KEGG orthology group: K02049, sulfonate/nitrate/taurine transport system ATP-binding protein (inferred from 97% identity to psa:PST_1824)

MetaCyc: 37% identical to L-glutamine ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-12-RXN [EC: 7.4.2.1]

Predicted SEED Role

"Hydroxymethylpyrimidine ABC transporter, ATPase component" in subsystem Thiamin biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNW8 at UniProt or InterPro

Protein Sequence (248 amino acids)

>Psest_2543 ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component (Pseudomonas stutzeri RCH2)
MQLRFEDVDKRFGQLEVIKGFSGEFAQGELVALVGPSGCGKSTLLHLVAGLEKPSAGRVL
ADGKPIAEPSPRRTLVFQEHALYPWLSLQGNVAMALELQGVAKSNAFEQAQSWLERVGLN
GFEHYYPHQVSGGMRQRTALARAFIAKPEVLLLDEPFGALDALTRMALQDVLRELIEEHQ
PTVLLVTHDVDEALYLADHVLVFSARPTRVLKTFNFTHCEKSHDLSDFAAERKEILSLLG
IKTEVTHP