Protein Info for HP15_248 in Marinobacter adhaerens HP15

Annotation: diaminopimelate epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 68% identical to DAPF_HAHCH: Diaminopimelate epimerase (dapF) from Hahella chejuensis (strain KCTC 2396)

KEGG orthology group: K01778, diaminopimelate epimerase [EC: 5.1.1.7] (inferred from 88% identity to maq:Maqu_0495)

MetaCyc: 61% identical to diaminopimelate epimerase (Escherichia coli K-12 substr. MG1655)
Diaminopimelate epimerase. [EC: 5.1.1.7]

Predicted SEED Role

"Diaminopimelate epimerase (EC 5.1.1.7)" in subsystem Lysine Biosynthesis DAP Pathway (EC 5.1.1.7)

MetaCyc Pathways

aspartate superpathway (23/25 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
L-lysine biosynthesis I (9/9 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
L-lysine biosynthesis VI (6/7 steps found)
L-lysine biosynthesis II (6/9 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PK81 at UniProt or InterPro

Protein Sequence (304 amino acids)

>HP15_248 diaminopimelate epimerase (Marinobacter adhaerens HP15)
MTQQRRTQGPMLRFTKMHGLGNDFMVVDAISQPFRLRPEMIRELANRNFGIGFDQLLVVE
PPGLPDVDFRYRIFNADGSEVEQCGNGARCFARFVRDQRLTNKKVIRVQTAKGVIELRVG
REGMVMVNMGVPEFNPPAIPFAADRRKDVYTVDVDGQTVELSALSMGNPHGVLLVEDVDQ
APVETLGPKLERHPRFPARANIGFLQILDRTHVRLRVFERGSGETLACGSGACAAVVAGC
LRGLLDARVEVELRGGPLVIEWQGEGTPVMMEGPATSVFEGQLRLPGDSGGRRRRSARPH
KQRS