Protein Info for Psest_0249 in Pseudomonas stutzeri RCH2

Annotation: UDP-N-acetylglucosamine 2-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 52% identical to Y1034_THEMA: Putative UDP-N-acetylglucosamine 2-epimerase (TM_1034) from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)

KEGG orthology group: K01791, UDP-N-acetylglucosamine 2-epimerase [EC: 5.1.3.14] (inferred from 63% identity to ppw:PputW619_2931)

Predicted SEED Role

"UDP-N-acetylglucosamine 2-epimerase (EC 5.1.3.14)" in subsystem CMP-N-acetylneuraminate Biosynthesis or Sialic Acid Metabolism (EC 5.1.3.14)

MetaCyc Pathways

CMP-N-acetylneuraminate biosynthesis II (bacteria) (3/3 steps found)
UDP-N-acetyl-α-D-mannosamine biosynthesis (1/1 steps found)
CMP-N-acetylneuraminate biosynthesis I (eukaryotes) (2/5 steps found)
superpathway of CMP-sialic acids biosynthesis (3/15 steps found)
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (9/24 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHG8 at UniProt or InterPro

Protein Sequence (396 amino acids)

>Psest_0249 UDP-N-acetylglucosamine 2-epimerase (Pseudomonas stutzeri RCH2)
MSINSLIVSNEGSSRPSFRPKVMLVFGTRPEAIKMAPLARALRDAEDLELCLCVTGQHRQ
MLDDVLRLFALTPDHDLAVMTPGQTLNGLSAAIVSRLDPVLEAEQPDMVLVHGDTTTSFA
AALAAFHRSIPVGHVEAGLRTGNLYAPWPEEANRRLTSVLAELHFAPTATSRDNLLGEGV
PAARIDVTGNTVIDALLWMRKTLHESGWKPEDPALASLPERARVVLVTGHRRENFGDGFL
RICRALRHLALRHPDVRFIYPVHLNPNVQEPVYRLLDGLPNLILIPPLSYQAFVYLMDRA
TVILTDSGGVQEEAPSLGKPVLVMRDTTERPEAVAAGTVRLVGTDEERIIEEVSRLLDDS
AAYEQMQHAHNPYGDGNACAHILDRLRKRLISGCAE