Protein Info for HP15_2423 in Marinobacter adhaerens HP15

Annotation: acyl-CoA thiolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00108: Thiolase_N
  • amino acids 1 to 249 (249 residues), 274.7 bits, E=7.6e-86
• TIGR01930: acetyl-CoA C-acyltransferase
  • amino acids 2 to 377 (376 residues), 415.5 bits, E=1e-128
• PF02803: Thiolase_C
  • amino acids 256 to 377 (122 residues), 140.9 bits, E=1.7e-45

Best Hits

Swiss-Prot: 48% identical to THLA_CLOAB: Acetyl-CoA acetyltransferase (thlA) from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)

KEGG orthology group: K00626, acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (inferred from 91% identity to maq:Maqu_2696)

MetaCyc: 55% identical to acetyl-CoA C-acetyltransferase (Methylibium petroleiphilum PM1)
Acetyl-CoA C-acyltransferase. [EC: 2.3.1.16, 2.3.1.9]

Predicted SEED Role

"3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9)" (EC 2.3.1.16, EC 2.3.1.9)

MetaCyc Pathways

• oleate β-oxidation (30/35 steps found)
• fatty acid salvage (6/6 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (5/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• L-isoleucine degradation I (5/6 steps found)
• (2S)-ethylmalonyl-CoA biosynthesis (3/4 steps found)
• pyruvate fermentation to hexanol (engineered) (8/11 steps found)
• acetyl-CoA fermentation to butanoate (5/7 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
• ketolysis (2/3 steps found)
• polyhydroxybutanoate biosynthesis (2/3 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• acetoacetate degradation (to acetyl CoA) (1/2 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• valproate β-oxidation (6/9 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• ketogenesis (3/5 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (4/7 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• 5,6-dehydrokavain biosynthesis (engineered) (6/10 steps found)
• propanoate fermentation to 2-methylbutanoate (3/6 steps found)
• glycerol degradation to butanol (10/16 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
• fatty acid β-oxidation VII (yeast peroxisome) (2/5 steps found)
• isopropanol biosynthesis (engineered) (2/5 steps found)
• pyruvate fermentation to acetone (2/5 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• 4-ethylphenol degradation (anaerobic) (2/6 steps found)
• 4-oxopentanoate degradation (4/9 steps found)
• ethylbenzene degradation (anaerobic) (1/5 steps found)
• 2-deoxy-D-ribose degradation II (3/8 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
• L-lysine fermentation to acetate and butanoate (4/10 steps found)
• superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (4/10 steps found)
• 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) (1/6 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• 2-methylpropene degradation (2/8 steps found)
• mevalonate pathway I (eukaryotes and bacteria) (1/7 steps found)
• mevalonate pathway II (haloarchaea) (1/7 steps found)
• 3-phenylpropanoate degradation (3/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• L-glutamate degradation VII (to butanoate) (4/12 steps found)
• isoprene biosynthesis II (engineered) (1/8 steps found)
• mevalonate pathway III (Thermoplasma) (1/8 steps found)
• mevalonate pathway IV (archaea) (1/8 steps found)
• ethylmalonyl-CoA pathway (3/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (8/18 steps found)
• methyl tert-butyl ether degradation (2/10 steps found)
• L-tryptophan degradation III (eukaryotic) (5/15 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (5/16 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (5/17 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• toluene degradation VI (anaerobic) (4/18 steps found)
• jasmonic acid biosynthesis (4/19 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
• platensimycin biosynthesis (7/26 steps found)
• sitosterol degradation to androstenedione (1/18 steps found)
• androstenedione degradation I (aerobic) (6/25 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
• superpathway of testosterone and androsterone degradation (6/28 steps found)
• androstenedione degradation II (anaerobic) (4/27 steps found)
• superpathway of ergosterol biosynthesis I (3/26 steps found)
• superpathway of L-lysine degradation (12/43 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
• superpathway of cholesterol biosynthesis (3/38 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (9/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (5/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Ethylbenzene degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Lysine degradation
• Propanoate metabolism
• Pyruvate metabolism
• Synthesis and degradation of ketone bodies
• Terpenoid biosynthesis
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.16, 2.3.1.9

Use Curated BLAST to search for 2.3.1.16 or 2.3.1.9

Sequence Analysis Tools

See E4PH84 at UniProt or InterPro

Protein Sequence (379 amino acids)

>HP15_2423 acyl-CoA thiolase (Marinobacter adhaerens HP15)
MGGFQGSLASVSATDLGAITIAEAVRRAGLQPADVQEVIMGNVLPAGLKQGPARQAMRKA
GLPDHTGATTINKLCGSGMKAAMFAHDLIKAGSNDIMVAGGMESMSNAPYILQGVRSGYR
MGPGQAPQDHMFLDGLEDAETGRLMGAFAQEMADKKGYTREEMDEYAITSLTRAKKAIEE
GLLKDEIIPVTVKSRKGEVVVEDDEQPHNANIDKIPSLRPAFAKDGTVTAANASSISDGA
SALLLMRESEAEKRGLKPLARIVGHSTQSQHPSEFTCAPVGAIETLFGKTGWSKDDVDLF
EINEAFAMVAMMPIRELGLDPEKVNIHGGACAQGHPVGSTGSRLLVTLMYALQRYGKKKG
IAALCIGGGEATAMAIEML