Protein Info for HP15_2414 in Marinobacter adhaerens HP15

Annotation: protein containing uncharacterized protein family UPF0066 domains

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 247 TIGR00104: tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase TsaA" amino acids 12 to 157 (146 residues), 140.6 bits, see alignment E=2e-45 PF01980: TrmO_N" amino acids 30 to 152 (123 residues), 134.8 bits, see alignment E=1.7e-43 PF18389: TrmO_C" amino acids 168 to 236 (69 residues), 72.5 bits, see alignment E=2.3e-24

Best Hits

Swiss-Prot: 49% identical to TRMO_ECOLI: tRNA (adenine(37)-N6)-methyltransferase (trmO) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 80% identity to maq:Maqu_2686)

MetaCyc: 49% identical to tRNA m6t6A37 methyltransferase (Escherichia coli K-12 substr. MG1655)
2.1.1.-

Predicted SEED Role

"COG1720: Uncharacterized conserved protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PH75 at UniProt or InterPro

Protein Sequence (247 amino acids)

>HP15_2414 protein containing uncharacterized protein family UPF0066 domains (Marinobacter adhaerens HP15)
MPRHPSKPATEALTLTPIAITRSCFQDKFGVPRQPGLTRHARAELIIRPPFDREDAFRGL
ETASHLWLTFQFHEAVRADWRPVVRPPRLGGNRKMGVFASRSPFRPNSLGLSVVRNEGLV
RKDGQLILRISDHDLIEGTPILDIKPYLPFADSVPEATLGWADSPPTEKLDVVFLPEAEA
QIGKLSPEEYPELRALIEDVVAYDPRPSFRRGREEERIYGAHLYDLNVRFRFVNDHSRKR
VEVLTVC