Protein Info for PGA1_c24560 in Phaeobacter inhibens DSM 17395

Annotation: carbamoyl-phosphate synthase large chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1103 (1102 residues), 1435.8 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 161 to 347 (187 residues), 214.9 bits, E=3.5e-67
  • amino acids 697 to 903 (207 residues), 105.3 bits, E=1.1e-33
• PF02787: CPSase_L_D3
  • amino acids 442 to 520 (79 residues), 80.4 bits, 2.7e-26
• PF02655: ATP-grasp_3
  • amino acids 702 to 874 (173 residues), 24.3 bits, E=1.1e-08
• PF02222: ATP-grasp
  • amino acids 705 to 874 (170 residues), 28.6 bits, E=3.7e-10
• PF02142: MGS
  • amino acids 1007 to 1092 (86 residues), 64.8 bits, 2.3e-21

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of arginine and polyamine biosynthesis (17/17 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-citrulline biosynthesis (8/8 steps found)
• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• superpathway of L-citrulline metabolism (10/12 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis I (5/6 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (4/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See I7DSU0 at UniProt or InterPro

Protein Sequence (1119 amino acids)

>PGA1_c24560 carbamoyl-phosphate synthase large chain (Phaeobacter inhibens DSM 17395)
MPKRTDIQSIMIIGAGPIIIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPGL
ADATYIEPITPEVVAKIIEKERPDALLPTMGGQTGLNTALALEEMGVLAKFDVEMIGAKR
EAIEMAEDRKLFREAMDRLGLENPRATIITAPKKDNGNADLDAGVQMALDELEDIGLPAI
IRPAFTLGGTGGGVAYNREDYIHFCRSGMDASPVNQILVDESLLGWKEYEMEVVRDTADN
AIIVCSIENIDPMGVHTGDSITVAPALTLTDKEYQMMRSASIAVLREIGVETGGSNVQWA
VNPADGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGFTLDELDNDITGVTPASF
EPTIDYVVTKIPRFAFEKFAGSEPYLTTAMKSVGETMAIGRTIHESLQKALASMETGLTG
FDEVAIPGAEDETDGKAAVIKAISQQTPDRMRTIAQAMRHGLSDDDIHAVTKFDPWFLAR
IREIVEAEAKIRAEGLPQDEHGLRAIKMLGFTDARLAMLTDQREGDIRAARRALGVNAVF
KRIDTCAAEFEAQTPYMYSTYEAPAFGDVECEARPSDKKKVVILGGGPNRIGQGIEFDYC
CCHACFALTDAGYETIMINCNPETVSTDYDTSDRLYFEPLTFEHVMEILTKELENGTLHG
VIVQFGGQTPLKLANALEEEGIPILGTSPDAIDLAEDRERFQALVNQLGLKQPHNGIAST
DAQALEIAEEIGFPLVIRPSYVLGGRAMEIVRDMDQLKRYIAEAVVVSGDSPVLLDSYLS
GAVELDVDAICDGTEVHVAGIMQHIEEAGVHSGDSACSLPPYSLSKEVIAEVKTQTNALA
KALNVVGLMNIQFAVKPDADGKDVIYLIEVNPRASRTVPFVAKSTDSAIASIAARVMAGE
PLSNFPKRAPYEPDAGYDVNVPMADPMTLADPDMPWFSVKEAVLPFARFPGVDTLLGPEM
RSTGEVMGWDRSFARAFLKAQMGAGMVLPSEGRAFISIKDADKGTLMLDAAKILVEQGFT
LVATRGTQSWLDGHGVPCELVNKVYEGRPHVVDMLKDGNVQLLMNTTEGAQAVQDSKDMR
SVALYDKIPYFTTAAGANAAARAIKAQAEGDVEVKSLQG