Protein Info for Psest_2438 in Pseudomonas stutzeri RCH2

Updated annotation (from data): 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31)
Rationale: Specifically important for utilizing L-Valine. Automated validation from mutant phenotype: the predicted function (3-HYDROXYISOBUTYRATE-DEHYDROGENASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: 3-hydroxyisobutyrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 transmembrane" amino acids 177 to 195 (19 residues), see Phobius details PF03807: F420_oxidored" amino acids 3 to 94 (92 residues), 25.7 bits, see alignment E=2.1e-09 PF03446: NAD_binding_2" amino acids 3 to 162 (160 residues), 180.2 bits, see alignment E=4.9e-57 TIGR01692: 3-hydroxyisobutyrate dehydrogenase" amino acids 5 to 293 (289 residues), 444.1 bits, see alignment E=1.1e-137 PF14833: NAD_binding_11" amino acids 165 to 290 (126 residues), 111.5 bits, see alignment E=4.8e-36

Best Hits

Swiss-Prot: 58% identical to 3HIDH_DICDI: Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial (hibA) from Dictyostelium discoideum

KEGG orthology group: K00020, 3-hydroxyisobutyrate dehydrogenase [EC: 1.1.1.31] (inferred from 97% identity to psa:PST_1924)

MetaCyc: 63% identical to 3-hydroxyisobutyrate dehydrogenase subunit (Pseudomonas putida)
3-hydroxyisobutyrate dehydrogenase. [EC: 1.1.1.31]

Predicted SEED Role

"3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.1.1.31)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.31

Use Curated BLAST to search for 1.1.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNL5 at UniProt or InterPro

Protein Sequence (296 amino acids)

>Psest_2438 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) (Pseudomonas stutzeri RCH2)
MHIGFIGLGNMGAPMAHNLLKAGHQLSVFDLNAAAVENLVGAGALPVDSPTAIAQGNAEL
IITMLPAAAHVKGVYLGVNGLIAHSRAGVMLIDCSTIDPHSAREVAKAAAEHGNPMLDAP
VSGGTGGAAAGTLTFMVGGSDPDFDHAQPILAAMGKNIVHCGAAGNGQVAKVANNMLLGI
SMIGVAEAMALGVALGMDAKTLAGVINTSSGRCWSSDTYNPFPGVLDNVPSSRGYSGGFG
SDLMLKDLGLATEAAKQVRQPVILGALAQQLYQSFSAQGHGGLDFSAIINQYRKDT