Protein Info for Psest_2392 in Pseudomonas stutzeri RCH2

Annotation: PAS domain S-box/diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 886 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 35 to 56 (22 residues), see Phobius details amino acids 68 to 89 (22 residues), see Phobius details amino acids 95 to 118 (24 residues), see Phobius details amino acids 130 to 153 (24 residues), see Phobius details amino acids 165 to 188 (24 residues), see Phobius details PF07694: 5TM-5TMR_LYT" amino acids 29 to 185 (157 residues), 76 bits, see alignment E=1.1e-24 TIGR00229: PAS domain S-box protein" amino acids 200 to 320 (121 residues), 29.1 bits, see alignment E=9e-11 amino acids 321 to 442 (122 residues), 69.6 bits, see alignment E=2.7e-23 PF00989: PAS" amino acids 203 to 312 (110 residues), 22.6 bits, see alignment E=3.6e-08 amino acids 331 to 433 (103 residues), 41.5 bits, see alignment E=5e-14 PF08448: PAS_4" amino acids 210 to 316 (107 residues), 53.7 bits, see alignment E=9.3e-18 amino acids 331 to 437 (107 residues), 32.4 bits, see alignment E=3.7e-11 PF13426: PAS_9" amino acids 333 to 435 (103 residues), 57.9 bits, see alignment E=4.3e-19 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 445 to 612 (168 residues), 141.8 bits, see alignment E=1.7e-45 PF00990: GGDEF" amino acids 447 to 608 (162 residues), 155.7 bits, see alignment E=3.6e-49 PF00563: EAL" amino acids 629 to 864 (236 residues), 243.8 bits, see alignment E=6.5e-76

Best Hits

KEGG orthology group: None (inferred from 80% identity to psa:PST_1969)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNL7 at UniProt or InterPro

Protein Sequence (886 amino acids)

>Psest_2392 PAS domain S-box/diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MTLNAVVENAVMLLALCWLLAFTTSEWYRSAKLSAKLLAGLWFGSACILGMMSTSFGESG
IMLDARTVVLSMAGLFGGPVVAGTAGVLAAGYRLWLGGVGVVPGLVNILLPILLGLTYRC
LHRQGLIRIGFWQLLGFGAMLHLGVLGVIISLLPDHLRFVAAQEAALPMLLALPLATAAL
GLLLNDLLKRDQVERALRLSEARLRAITHAIPDLLLVIDEDGCYLDIICADHSMLYADPS
LLLGRHLRDMMPEAEERRFLDFIRQTLNSDTPQLIEYSMQTLGGPRIFEGRALPLEQPPG
QKRAVVWLSRDITDRVNTELERRIAAIAFESQQGMLITDGQNRILRVNRAFSRISGFSAE
EAVGKTTALLASGKHGSEFYRAMWASIEATGVWEGEIWNRRKSGEIFPEWLTISAVRNLT
GDISHYVAAFTDITDRKAAEERIHNLAFYDPLTGLPNRRLLLDRLQQAMAAGCRSGQLGA
LMFIDLDNFKNINDLHGHQTGDQVLRIAAERLQGEIRASDTVARLGGDEFVVMLENLGDA
PELAATQAEHIGMKLLGSLGKPYRLNDLGLYSSASIGVVLFGTDGSSSDELMKRADMSMY
EAKVAGKNALRFFDPRMQQAVQERLHLENEIRQGLKNGEFILHYQAQLEQSQGIVGAEAL
VRWLHPQRGLLAPGAFIGQAEHAGLIQTLDQHVLEQACAELALWAKQPALAQLTLSVNIS
ANLLYENNFVGSLLELLESSGANPARLKLELTETLLLDNMPEAIARMTQLKNHGIRFSID
DFGTGYSSMSYLQQLPLDQLKIDQSFVRGLPEDANSLTIVRATCALAAGLHLEVIAEGVE
NEAQRTMLLANGCHRFQGYLFGKPQAAASFEEMVMASRAEHSSFCS