Protein Info for Psest_2340 in Pseudomonas stutzeri RCH2

Annotation: Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00561: Abhydrolase_1
  • amino acids 35 to 258 (224 residues), 86 bits, E=1.7e-28

Best Hits

Swiss-Prot: 76% identical to LIP_PSEAE: Triacylglycerol lipase (lip) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01046, triacylglycerol lipase [EC: 3.1.1.3] (inferred from 95% identity to psa:PST_2016)

Predicted SEED Role

"Lipase precursor (EC 3.1.1.3)" in subsystem Cytolysin and Lipase operon in Vibrio (EC 3.1.1.3)

MetaCyc Pathways

• triacylglycerol degradation (1/3 steps found)
• retinol biosynthesis (1/7 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.3

Use Curated BLAST to search for 3.1.1.3

Sequence Analysis Tools

See L0GLJ3 at UniProt or InterPro

Protein Sequence (311 amino acids)

>Psest_2340 Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold (Pseudomonas stutzeri RCH2)
MNKNKTLLALCLGSALVLTGQAYAASGSGYTATKYPIVLAHGMLGFDSLLGIDYWYGIPK
ALRRDGAQVYVTEVSQLNTSELRGEELLAQVEEIVAISGKPKVNLIGHSHGGPTVRYVAG
VRPDLIASVTSVGAPHKGSDVADLIRKIPEGSSGEAIVAGLVNAMGTFINFLSGSSSSAP
QDSLGSLESLNSEGAARFNAKFPQGIPTTACGEGAYKVNGVHYYSWSGTSPLTNALDISD
AMMGAGSLAFSGPNDGLVGRCSSHLGMVIRDNYRMNHLDEVNQFMGLTSLFETDPVSVYR
QHANRMKNAGL