Protein Info for HP15_2236 in Marinobacter adhaerens HP15

Annotation: glutamyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 473 transmembrane" amino acids 412 to 430 (19 residues), see Phobius details PF00749: tRNA-synt_1c" amino acids 2 to 297 (296 residues), 313.7 bits, see alignment E=1.2e-97 TIGR00464: glutamate--tRNA ligase" amino acids 2 to 446 (445 residues), 414.8 bits, see alignment E=2.5e-128 PF19269: Anticodon_2" amino acids 311 to 449 (139 residues), 135.2 bits, see alignment E=2.3e-43

Best Hits

Swiss-Prot: 94% identical to SYE_MARHV: Glutamate--tRNA ligase (gltX) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 94% identity to maq:Maqu_1891)

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17) @ Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)" (EC 6.1.1.17, EC 6.1.1.24)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.17 or 6.1.1.24

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PFT0 at UniProt or InterPro

Protein Sequence (473 amino acids)

>HP15_2236 glutamyl-tRNA synthetase (Marinobacter adhaerens HP15)
MALFNLCFARQHGGQFILRIEDTDQARSTAESEQDILKALRWLGLNWDEGPDVGGPHGPY
RQSERKHSYRQYAEDLVTAGHAFYCFRTPDELEAIREERKAQGLNPGIKGDLELPEDEVK
RRLDAGEPYVIRMKVPDEGVCEIDDMLRGTIEIDWAQVDCQILLKSDGMPTYHLANVVDD
HLMEITHVLRGEEWINSAPKHKLLYQYFGWDMPVLCHLPLLRNPDKSKLSKRKNPTSINF
YERMGFLPEAVTNYLGRMGWSMPDEREKFTLDEMIENFDIQRVSLGGPVFDVEKLRWLNG
QWLREELTEEQFMERMRQWWFNEDALRALVPHIKGRAEVFSDVAPMAQFMFSGMLNLRPE
DFVHNKLEEAQIKRVLQFTLWKLEAQRHWSKETIFADIKALAKAMDLKMGDFMFSIFVAV
AGTPNSWSVMDSMALLGPDMTRSRLRHALEVMGGFSKKETKKVEKEYAALGIH