Protein Info for Psest_2316 in Pseudomonas stutzeri RCH2

Annotation: phosphoenolpyruvate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 5 to 786 (782 residues), 1213 bits, E=0
• PF01326: PPDK_N
  • amino acids 17 to 345 (329 residues), 425.7 bits, E=2.1e-131
• PF00391: PEP-utilizers
  • amino acids 384 to 454 (71 residues), 100.7 bits, E=4.1e-33
• PF02896: PEP-utilizers_C
  • amino acids 481 to 780 (300 residues), 202.7 bits, E=1.1e-63

Best Hits

Swiss-Prot: 91% identical to PPSA_PSEAB: Phosphoenolpyruvate synthase (ppsA) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 89% identity to avn:Avin_23280)

MetaCyc: 69% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (16/19 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• superpathway of hexitol degradation (bacteria) (13/18 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of N-acetylneuraminate degradation (14/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See L0GM23 at UniProt or InterPro

Protein Sequence (789 amino acids)

>Psest_2316 phosphoenolpyruvate synthase (Pseudomonas stutzeri RCH2)
MVEYVVSLDKLGNHDVERVGGKNASLGEMISNLAGAGVSVPGGFATTAQAYRDFMELSGL
NEQIHALLDALDVDDVNALAKAGAQIRGWVMEAQFPPQLDADIRTAFAEMSGGNDNMAVA
VRSSATAEDLPDASFAGQQETFLNIRGVDNVIRATKEVFASLFNDRAIAYRVHQGFDHKL
VALSAGVQRMVRSETGTAGVMFTLDTESGFRDVVFITGAYGLGETVVQGAVNPDEFYVHK
HTLEAGRPAILRRNLGSKAIKMVYGAEASAGKSVKTVDVDQADRMRFCLTDEEVTNLAKQ
AMTIEKHYGRPMDIEWAKDGDDNQLYIVQARPETVKSRSSANVMERYLLKEKGTVLVEGR
AIGQRIGAGPVKVIHDVSEMDKVQPGDVLVSDMTDPDWEPVMKRASAIVTNRGGRTCHAA
IIARELGIPAVVGCGNATELLKDGQRVTVSCAEGDTGLIFDGELGFDIRQNSIDAMPELP
FKIMMNVGNPDRAFDFAHLPNEGVGLARLEFIINRMIGVHPKALLNFEGLPADVKSSVEK
RIAGYDDPVNFYVEKLVEGVSTLAAAFWPKKVIVRLSDFKSNEYANLIGGKLYEPEEENP
MLGFRGASRYISDSFRDCFELECRAMKKVRDVMGLTNVELMVPFVRTLGEASQVIDLLAK
YGLKRGENGLRVIMMCELPSNALLADEFLEFFDGFSIGSNDMTQLTLGLDRDSGIIAHLF
DERNPAVKKLLANAIQACNKAGKYIGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDT
WFFLADREI