Protein Info for Psest_2305 in Pseudomonas stutzeri RCH2

Annotation: UDP-2,3-diacylglucosamine hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00149: Metallophos
  • amino acids 2 to 198 (197 residues), 35.2 bits, E=1.8e-12
• TIGR01854: UDP-2,3-diacylglucosamine diphosphatase
  • amino acids 3 to 231 (229 residues), 329.2 bits, E=5.6e-103

Best Hits

Swiss-Prot: 88% identical to LPXH_PSEU5: UDP-2,3-diacylglucosamine hydrolase (lpxH) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K03269, UDP-2,3-diacylglucosamine hydrolase [EC: 3.6.1.-] (inferred from 88% identity to psa:PST_2050)

MetaCyc: 48% identical to UDP-2,3-diacylglucosamine diphosphatase (Escherichia coli K-12 substr. MG1655)
LIPIDXSYNTHESIS-RXN [EC: 3.6.1.54]

Predicted SEED Role

"UDP-2,3-diacylglucosamine diphosphatase (EC 3.6.1.54)" (EC 3.6.1.54)

MetaCyc Pathways

• lipid IV_A biosynthesis (P. gingivalis) (9/9 steps found)
• superpathway of (Kdo)₂-lipid A biosynthesis (14/17 steps found)
• lipid IV_A biosynthesis (E. coli) (6/6 steps found)
• lipid IV_A biosynthesis (H. pylori) (6/6 steps found)
• lipid IV_A biosynthesis (P. putida) (6/6 steps found)
• lipid IV_A biosynthesis (Vibrio cholerae serogroup O1 El Tor) (6/6 steps found)
• lipid IV_A biosynthesis (generic) (6/6 steps found)
• lipid IV_A biosynthesis (2,3-diamino-2,3-dideoxy-D-glucopyranose-containing) (5/6 steps found)
• superpathway of Kdo₂-lipid A biosynthesis (17/25 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Lipopolysaccharide biosynthesis
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-

Use Curated BLAST to search for 3.6.1.- or 3.6.1.54

Sequence Analysis Tools

See L0GLF3 at UniProt or InterPro

Protein Sequence (240 amino acids)

>Psest_2305 UDP-2,3-diacylglucosamine hydrolase (Pseudomonas stutzeri RCH2)
MILLISDLHLEEERPDISRAFLHFLETRACQAEALYILGDFFEVWIGDDAMTPFQRSIAQ
ALRSLSGSGTRIYLMHGNRDFMLGKSFCRAAGCTLLADPSVVELNGERVLLMHGDSLCTR
DEGYMRLRRLLRNPFSLFVLRNLPLSARRKLARKLRNESRTQTRMKASDIIDVTPELIPR
VLAEHRVRTLIHGHTHRPAMHDLVVNGQPAQRIVLGDWDRQGWALQVDESGFHQAPFELS