Protein Info for Psest_2303 in Pseudomonas stutzeri RCH2

Annotation: glutaminyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 576 PF00749: tRNA-synt_1c" amino acids 49 to 342 (294 residues), 362.6 bits, see alignment E=2.2e-112 TIGR00440: glutamine--tRNA ligase" amino acids 51 to 574 (524 residues), 781.3 bits, see alignment E=2.7e-239 PF03950: tRNA-synt_1c_C" amino acids 362 to 461 (100 residues), 102.1 bits, see alignment E=2.5e-33 PF20974: tRNA-synt_1c_C2" amino acids 477 to 553 (77 residues), 92.6 bits, see alignment E=3.2e-30

Best Hits

Swiss-Prot: 97% identical to SYQ_PSEU5: Glutamine--tRNA ligase (glnS) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01886, glutaminyl-tRNA synthetase [EC: 6.1.1.18] (inferred from 97% identity to psa:PST_2052)

MetaCyc: 62% identical to glutamine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glutamine--tRNA ligase. [EC: 6.1.1.18]

Predicted SEED Role

"Glutaminyl-tRNA synthetase (EC 6.1.1.18)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNC6 at UniProt or InterPro

Protein Sequence (576 amino acids)

>Psest_2303 glutaminyl-tRNA synthetase (Pseudomonas stutzeri RCH2)
MISALIWSAYPEPHSLIVKDIMSKPETPAASNFLRPIVQTDLDSGKHAKIVTRFPPEPNG
YLHIGHAKSICLNFGLAKEFGGECNLRFDDTNPAKEDQEYIDAIKTDVEWLGFQWAGEER
YASNYFDQLHEWAVHLIKAGKAYVCDLTSEQAREYRGSLTEPGRNSPFRERSVEENLDLF
ARMKAGEFPDGARALRAKIDMASPNMNLRDPILYRIRHAHHHQTGDKWCIYPSYDFTHGQ
SDAIEGITHSICTLEFEDHRPLYEWFLANLPVPAQPRQYEFARLNLNYTITSKRKLKQLV
DEKHVSGWDDPRMSTLSGFRRRGYTPASIRNFCDMIGVNRAGGVVDIGMLEFAIREDLDA
NAARAMCVLKPLKVVITNYPEGQVENLELPRHPKQDMGVRVLPFSREIYIDTSDFEEVPP
AGFKRLIPGGEVRLRGSYVIRADEAVKDEAGNIVELRCSYDDNTLGKNPEGRKVKGVIHW
VPAAESVECEVRLYDRLFRSANPEKDEEGGSFLDNINPESLVVLKGCRAEPSLANAEPEE
RFQFEREGYFCADLKDSKPGAPVFNRTVTLRDSWGQ