Protein Info for Psest_2302 in Pseudomonas stutzeri RCH2

Annotation: cysteinyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 462 TIGR00435: cysteine--tRNA ligase" amino acids 4 to 459 (456 residues), 588.5 bits, see alignment E=5.7e-181 PF01406: tRNA-synt_1e" amino acids 17 to 315 (299 residues), 460 bits, see alignment E=1e-141 PF09334: tRNA-synt_1g" amino acids 38 to 137 (100 residues), 25.9 bits, see alignment E=1.1e-09 amino acids 248 to 310 (63 residues), 25.2 bits, see alignment E=1.8e-09 PF00133: tRNA-synt_1" amino acids 227 to 305 (79 residues), 25.6 bits, see alignment E=1.3e-09 PF09190: DALR_2" amino acids 342 to 403 (62 residues), 80.8 bits, see alignment E=2.2e-26

Best Hits

Swiss-Prot: 94% identical to SYC_PSEU5: Cysteine--tRNA ligase (cysS) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 94% identity to psa:PST_2053)

MetaCyc: 62% identical to cysteine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJA0 at UniProt or InterPro

Protein Sequence (462 amino acids)

>Psest_2302 cysteinyl-tRNA synthetase (Pseudomonas stutzeri RCH2)
MMALSIYNTLTKTKEAFRPLQGNNVRMYVCGMTVYDFCHIGHARVMVAFDVVTRWLRHRG
YDVTYVRNITDIDDKIIRRAGENGEPFQALVERMIAAMHEDETRLDVLRPDIEPRATDHI
AGMHQMIQTLIDKGFAYAPGNGDVYYRVGKFVGYGKLSRRKIEDLKIGARIEVDEAKQDP
LDFVLWKGAKPGEPSWSSPWGAGRPGWHIECSVMSTCCLGETFDIHGGGPDLVFPHHENE
IAQSEAATGKQYANAWMHAGAVRVDGEKMSKSLGNFFTIREVLEKYHPEVVRYLLVSSHY
RSPINYSEDSLREAKSALERFYNGLKGLPNVAPAGGDEFVARFATAMDDDFNSPEACAVL
FDMIREVNRLRESDPTAAAQLAARLKELASVLGVLQLEPEAFLQAGAAGKVDAAEVEALI
AARLTARAEKNWAESDRIRDQLTAMGVVLEDGKGGTTWRLAE