Protein Info for Psest_2294 in Pseudomonas stutzeri RCH2

Annotation: nitrite reductase, copper-containing

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 459 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF00127: Copper-bind" amino acids 94 to 138 (45 residues), 28.7 bits, see alignment 3.8e-10 TIGR02376: nitrite reductase, copper-containing" amino acids 153 to 449 (297 residues), 328.9 bits, see alignment E=1.9e-102 PF07732: Cu-oxidase_3" amino acids 183 to 289 (107 residues), 55 bits, see alignment E=2.1e-18 PF07731: Cu-oxidase_2" amino acids 199 to 289 (91 residues), 31 bits, see alignment E=5e-11 amino acids 335 to 439 (105 residues), 23.9 bits, see alignment E=7.5e-09

Best Hits

KEGG orthology group: K00368, nitrite reductase (NO-forming) [EC: 1.7.2.1] (inferred from 70% identity to put:PT7_1499)

Predicted SEED Role

"Copper-containing nitrite reductase (EC 1.7.2.1)" in subsystem Denitrification (EC 1.7.2.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.2.1

Use Curated BLAST to search for 1.7.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJ96 at UniProt or InterPro

Protein Sequence (459 amino acids)

>Psest_2294 nitrite reductase, copper-containing (Pseudomonas stutzeri RCH2)
MRYLSRVLLLALLVLPNALWAQTKSSSDVSYRPDVTITLRTDIADGKLVYVSESGLTRGQ
VNPDLRVPEGAVVQINLINGDGAVHDIAVPEFEATSSEISGKGAATSIVFRATRNGTFEY
YCTLPGHKAAGMVGKLVVGDGPETVVEQGKDLSKDPTQVGEPVGNREPKSITLDLRTTEE
EGRLSDGSTYKFWTFDGTVPGPMVRIREGDTVTLNLSNEPDSAHIHSIDLHAVTGPGGGA
AVTQVAPGQTRSFTFRALQPGLYVYHCATPMVAQHISNGMYGLILVEPEGGLPKVDHEFY
VMQGELYTASPRGARGLHEFSLDMLLRETPQHLMFNGSKDALTSIHKMEVNTGDSVRIFF
GVGGPNLISSFHVIGEIFDKVFDQGSLTSPPLTDVQTTLVPAGGATMVEFVADYPGKYIL
VDHALSRAEKGLMGVLSVKGEADASIFSSPEPVDPHSGH