Protein Info for Psest_2286 in Pseudomonas stutzeri RCH2

Annotation: endopeptidase Clp ATP-binding regulatory subunit (clpX)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 426 TIGR00382: ATP-dependent Clp protease, ATP-binding subunit ClpX" amino acids 12 to 412 (401 residues), 685.7 bits, see alignment E=1.2e-210 PF06689: zf-C4_ClpX" amino acids 15 to 51 (37 residues), 70.7 bits, see alignment 3e-23 PF00493: MCM" amino acids 70 to 194 (125 residues), 24.7 bits, see alignment E=4.8e-09 PF01078: Mg_chelatase" amino acids 111 to 138 (28 residues), 21.5 bits, see alignment (E = 5.6e-08) PF07724: AAA_2" amino acids 113 to 311 (199 residues), 120.3 bits, see alignment E=3.7e-38 PF07728: AAA_5" amino acids 115 to 192 (78 residues), 26.3 bits, see alignment E=2.7e-09 PF00004: AAA" amino acids 116 to 222 (107 residues), 57.3 bits, see alignment E=9.6e-19 PF10431: ClpB_D2-small" amino acids 317 to 395 (79 residues), 46.1 bits, see alignment E=1.6e-15

Best Hits

Swiss-Prot: 94% identical to CLPX_PSESM: ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K03544, ATP-dependent Clp protease ATP-binding subunit ClpX (inferred from 99% identity to psa:PST_2062)

MetaCyc: 78% identical to ATP-dependent Clp protease ATP-binding subunit ClpX (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpX" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GN57 at UniProt or InterPro

Protein Sequence (426 amino acids)

>Psest_2286 endopeptidase Clp ATP-binding regulatory subunit (clpX) (Pseudomonas stutzeri RCH2)
MTDTRNGEDSGKLLYCSFCGKSQHEVRKLIAGPSVFICDECVDLCNDIIREEVQEAQAES
NAHKLPAPKEISGILDQYVIGQERAKKILAVAVYNHYKRLNQRDKKEEVELGKSNILLIG
PTGSGKTLLAETLARLLNVPFTIADATTLTEAGYVGEDVENIIQKLLQKCDYDVEKAQMG
IVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVASVPPQGGRKHPQQEFLQVD
TRNILFICGGAFAGLEKVIQGRSTQGGIGFNAEVRSKDPGKKIGEALRAVEPDDLVKFGL
IPEFVGRLPVIATLDELDEAALIQILTEPKNALTKQYAKLFELEGVDLEFRPDALKAIAS
RALERKTGARGLRSILEGVLLDTMYEIPSQKDVSKVVIDESVIEGTSQPLLIYENTEPPA
KAAPEA