Protein Info for HP15_2191 in Marinobacter adhaerens HP15

Annotation: glutaminyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 559 PF00749: tRNA-synt_1c" amino acids 28 to 338 (311 residues), 379.1 bits, see alignment E=2.2e-117 TIGR00440: glutamine--tRNA ligase" amino acids 29 to 556 (528 residues), 772.3 bits, see alignment E=1.5e-236 PF03950: tRNA-synt_1c_C" amino acids 341 to 441 (101 residues), 110.7 bits, see alignment E=5e-36 PF20974: tRNA-synt_1c_C2" amino acids 457 to 532 (76 residues), 72.8 bits, see alignment E=4.7e-24

Best Hits

Swiss-Prot: 65% identical to SYQ_PSEU5: Glutamine--tRNA ligase (glnS) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01886, glutaminyl-tRNA synthetase [EC: 6.1.1.18] (inferred from 92% identity to maq:Maqu_1845)

MetaCyc: 59% identical to glutamine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glutamine--tRNA ligase. [EC: 6.1.1.18]

Predicted SEED Role

"Glutaminyl-tRNA synthetase (EC 6.1.1.18)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PF74 at UniProt or InterPro

Protein Sequence (559 amino acids)

>HP15_2191 glutaminyl-tRNA synthetase (Marinobacter adhaerens HP15)
MSAESKKAHNFIQSLIEDAIASGEHTGKVVTRFPPEPNGYLHIGHAKSICLNFGIAETFS
GDCNLRFDDTNPEKENQEYIDAIKQDVEWLGYEWAGDVRYASDYFDALYEFAEELITRDK
AYVCALTADEMAEYRGSLKEPGRNSPYRDRPVEESLQLFRDMRDGKYQNGELVLRAKIDM
ASPNINMRDPILYRIRYAEHHQTGNKWCIYPMYDFTHPISDAMEGITHSLCTLEFEDHRP
LYDWVLDNISGPCHPRQIEFARLNLNYTITSKRKLKRLVDENVVDGWNDPRMPTISGMRR
RGYTPESIRTFCDMIGVNKAGGTVDVGMLEHAIREDLNTRAPRAMCVMRPLKVTLTNYPA
DQSETLTLPVHPQNPDMGEREVPWTQTLYIDREDFEMEPPRKWKRLAPDQAVRLRGGYVM
TCREVIRDDSGQIVELKCEYDPNTLGVNPEGYKPNGVIHWVSASDSVEADINLYDRLFNH
ESPDSDKEGDLMDHLNPESLVVLKGARVEKSLVAPRMDLPYQFEREGYFFCDQAHTEAAG
RPVFNRTVTLRDSWGKGGK