Protein Info for HP15_2187 in Marinobacter adhaerens HP15

Annotation: methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 21 to 136 (116 residues), 142 bits, E=1e-45
• PF02882: THF_DHG_CYH_C
  • amino acids 139 to 295 (157 residues), 230.4 bits, E=7e-73

Best Hits

Swiss-Prot: 92% identical to FOLD_MARHV: Bifunctional protein FolD (folD) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 90% identity to maq:Maqu_1842)

MetaCyc: 64% identical to bifunctional methylenetetrahydrofolate dehydrogenase / methenyltetrahydrofolate cyclohydrolase (Escherichia coli K-12 substr. MG1655)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• folate transformations III (E. coli) (8/9 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• folate transformations II (plants) (9/11 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• folate transformations I (8/13 steps found)
• L-histidine degradation III (1/6 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (2/10 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• purine nucleobases degradation II (anaerobic) (11/24 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Sequence Analysis Tools

See E4PF71 at UniProt or InterPro

Protein Sequence (300 amino acids)

>HP15_2187 methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase (Marinobacter adhaerens HP15)
MPTLQFTDAQPTRRDMTAKLINGKEIAAEVRQQVAAGVEARTQQGLRAPGLAVVLVGNDP
ASHVYVGNKRKACDQAGILSLSYDLPEDTSQEALEALIDELNENPTVDGILVQLPLPSHL
DADPILVKIRPDKDVDGFHPYNIGRLMQRKPTLRPCTPAGIITLLDSIDTPYKGQHAVIV
GASNIVGRPMSMELLLKGATTTVCHRFTPDLEKFVREADILVAAVGKPGLIKGDWVKPGA
TVIDVGINRMDDGKLHGDVDFAAASERAAYITPVPGGVGPMTIATLLQNTLYAADVLHRD