Protein Info for Psest_2223 in Pseudomonas stutzeri RCH2

Annotation: cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 249 PF00027: cNMP_binding" amino acids 45 to 130 (86 residues), 57.6 bits, see alignment E=1.5e-19 PF13545: HTH_Crp_2" amino acids 162 to 234 (73 residues), 61.6 bits, see alignment E=8.2e-21 PF00325: Crp" amino acids 187 to 218 (32 residues), 47.7 bits, see alignment 1.5e-16

Best Hits

Swiss-Prot: 39% identical to FNR_PASMU: Anaerobic regulatory protein (fnr) from Pasteurella multocida (strain Pm70)

KEGG orthology group: None (inferred from 41% identity to abo:ABO_1341)

Predicted SEED Role

"transcriptional regulator, Crp/Fnr family" in subsystem Oxidative stress

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GL69 at UniProt or InterPro

Protein Sequence (249 amino acids)

>Psest_2223 cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases (Pseudomonas stutzeri RCH2)
MDARKDADQAASLKCRECALRGVCLPATLLDPDLRQLEDIIRHNRRVARHKHLTRASDPA
NQVYALRSGTLKTYLTGSDGRELVTGFVFPGELVGLDAFAGGRSASHVMALEPSLVCAIP
VGELEDLLTANAKLRTRLFKFISHELQVEQDHLRHSREGAGQRFMAFFLDLSARHARRGL
SPTRFSLPMTRAEIGSYLGLTTETVSRLFTRYRQAGLIETRGREVQLLDVSSLAVAHAAP
ASRISCMAG