Protein Info for Psest_2219 in Pseudomonas stutzeri RCH2
Annotation: pyruvate dehydrogenase E1 component, alpha subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 45% identical to ODPA_ZYMMO: Pyruvate dehydrogenase E1 component subunit alpha (pdhA) from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)
KEGG orthology group: K00161, pyruvate dehydrogenase E1 component subunit alpha [EC: 1.2.4.1] (inferred from 75% identity to avn:Avin_46170)MetaCyc: 42% identical to pyruvate dehydrogenase complex E1 component alpha subunit (Synechococcus elongatus PCC 7942 = FACHB-805)
Predicted SEED Role
"Acetoin dehydrogenase E1 component alpha-subunit (EC 1.2.4.-)" in subsystem Acetoin, butanediol metabolism (EC 1.2.4.-)
MetaCyc Pathways
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
- pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Butanoate metabolism
- Citrate cycle (TCA cycle)
- Glycolysis / Gluconeogenesis
- Lysine degradation
- Pyruvate metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 1.2.4.-, 1.2.4.1
Use Curated BLAST to search for 1.2.4.- or 1.2.4.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GJ28 at UniProt or InterPro
Protein Sequence (330 amino acids)
>Psest_2219 pyruvate dehydrogenase E1 component, alpha subunit (Pseudomonas stutzeri RCH2) MNTQATLSYPPELALNLLRDMLRVRIMEERAAELYGEGKIRGFLHLYIGEEAVAVGALHA LAADDAIVATYREHGHALIQGVSMRAIMAEMYGRQQGCSGGRGGSMHLFDAGTRFFGGNA IVGGGLPLAVGLALAEQMRQGTRVSACFFGEGAMAEGAFHESMNLAALWQLPVLFCCENN LYAMGTALARSQSQTDLCAKAAAYRVAARAVDGMDVVAVHDATRAAVEHVRSGQGPYFLE LQTYRFRAHSMFDPELYRDKAEVELWKQRDPIHSYSVRLQAQGLLDAAGLLAIEAAVREE VEDAVAYAEAGTLEPVEQLCNDVYTPQVQP