Protein Info for Psest_0217 in Pseudomonas stutzeri RCH2

Annotation: diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 404 transmembrane" amino acids 53 to 73 (21 residues), see Phobius details amino acids 80 to 104 (25 residues), see Phobius details amino acids 115 to 134 (20 residues), see Phobius details amino acids 148 to 184 (37 residues), see Phobius details amino acids 193 to 213 (21 residues), see Phobius details TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 229 to 393 (165 residues), 143.7 bits, see alignment E=2.2e-46 PF00990: GGDEF" amino acids 231 to 389 (159 residues), 148.5 bits, see alignment E=7.6e-48

Best Hits

KEGG orthology group: None (inferred from 85% identity to psa:PST_4029)

Predicted SEED Role

"GGDEF family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GDK3 at UniProt or InterPro

Protein Sequence (404 amino acids)

>Psest_0217 diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MPHTTDYSSTEGFRDTPYGRQLHAGFRLLCFKSELEREFRHYLDRHARASQRLAALLLIL
VVSGYLYCELRLFDLAGTGWLTTLTLLRGLQILPGVVVLVLTFYSRRFRAQANRIFPVLL
VTVGILAALIDIRFEALQTELNFRYGAGLLIVSSFFFLGVTFWWALLCAVLIVLADVCMA
SLILPAERMPEHWIAVSYYVLLLIIGAISRYVHEYSQREQFLMRKLLGWVAEHDALSGLA
NRRSHDLALRQRVAQARRDRRPLSLLLLDLDDFKAYNDTFGHPAGDALIRTFADLLVGFA
RRPLDQAARVGGEEFALLLYNCDNAAAQRIARQLISALAALDIRHPQEAIARVGVSIGIA
TLQEGQLPEQLYHCADTALYQAKTSGKNRFAVAAAQPRSDLPDR