Protein Info for Psest_2172 in Pseudomonas stutzeri RCH2

Annotation: glycogen/starch synthases, ADP-glucose type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 516 TIGR02095: glycogen/starch synthase, ADP-glucose type" amino acids 39 to 505 (467 residues), 492.2 bits, see alignment E=7.3e-152 PF08323: Glyco_transf_5" amino acids 39 to 273 (235 residues), 234.4 bits, see alignment E=3.9e-73 PF13579: Glyco_trans_4_4" amino acids 54 to 260 (207 residues), 35.7 bits, see alignment E=2.8e-12 PF13439: Glyco_transf_4" amino acids 160 to 260 (101 residues), 29.7 bits, see alignment E=1.6e-10 PF00534: Glycos_transf_1" amino acids 330 to 471 (142 residues), 58.7 bits, see alignment E=1.4e-19 PF13692: Glyco_trans_1_4" amino acids 331 to 468 (138 residues), 57.3 bits, see alignment E=5.5e-19

Best Hits

Swiss-Prot: 67% identical to GLGA_PSESM: Glycogen synthase (glgA) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00703, starch synthase [EC: 2.4.1.21] (inferred from 89% identity to psa:PST_2137)

Predicted SEED Role

"Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21)" in subsystem Glycogen metabolism (EC 2.4.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GL23 at UniProt or InterPro

Protein Sequence (516 amino acids)

>Psest_2172 glycogen/starch synthases, ADP-glucose type (Pseudomonas stutzeri RCH2)
MSIAAVNARPAGTPKAQPAPPLVTRPALQIRSSHDDKRKILFVTSELTDLVKTGGLADVS
AALPRALGARHDVRVLIPGFSQVINSGHAIRIVGSLSSYAEIPGCRIGRMDMPDGLIIYV
LICPELYEREGSPYGDVHGNDWPDNPVRFARLGLAAAEIAAGTACIRWAPELVHAHDWPA
GLTPAYMRWRGLNTPSVFTIHNLAYQGNIDMSQRRLLGIPAEACDPERMEFYGKLSLLKA
GIAYANRVTTVSATYAEEITTPEFGCGMEGFLSMKARQGLLSGLLNGIDESWAPESDPYL
VSGFSARNWAGKEANAAYIRNSFGLEQCGAPLFAVVSRLVHQKGVDLTLDVAHAIVEGGG
QLAILGQGDHHIEDEVRQLAAQYPGRVAAHIGFNETDARRLFAGSDFLLMPSRYEPCGLS
QMYAQRYASLPIARRTGGLADSIEDGVTGFLFNEPDVASYRQAVQRALSIHQHPQLLSAM
RCRAMAAGFFWRHAIEPYDALYQQLLGERREVRHLI