Protein Info for Psest_2171 in Pseudomonas stutzeri RCH2

Annotation: malto-oligosyltrehalose trehalohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 transmembrane" amino acids 402 to 420 (19 residues), see Phobius details PF02922: CBM_48" amino acids 12 to 71 (60 residues), 23.9 bits, see alignment 6.6e-09 TIGR02402: malto-oligosyltrehalose trehalohydrolase" amino acids 21 to 553 (533 residues), 685.3 bits, see alignment E=2.5e-210 PF00128: Alpha-amylase" amino acids 126 to 222 (97 residues), 41.8 bits, see alignment E=1.3e-14 PF11941: DUF3459" amino acids 497 to 583 (87 residues), 70 bits, see alignment E=2.7e-23

Best Hits

KEGG orthology group: K01236, maltooligosyltrehalose trehalohydrolase [EC: 3.2.1.141] (inferred from 86% identity to psa:PST_2138)

Predicted SEED Role

"Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)" in subsystem Trehalose Biosynthesis (EC 3.2.1.141)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GMU4 at UniProt or InterPro

Protein Sequence (591 amino acids)

>Psest_2171 malto-oligosyltrehalose trehalohydrolase (Pseudomonas stutzeri RCH2)
MQKQRAHIGHHGPELLEDGNTRFRLWAPDAQNVSLIVVGAQTLPMTATEEGWYSIDAPYG
AGTLYRFLIDDELHVPDPASRAQAGDVHAPSLVIDTHNNYLWRNADWRGRPWHETVLYET
HVGLYGGFAAMEEHLAALAELGVTAIELMPIAEFPGDRNWGYDGVLPYAPEAAYGSPEEL
KHLIDTAHGLGLMVFLDVVYNHFGPDGNYLGRYAKHFFRHDQQTPWGDGIDFRRREVRDF
FIDNALMWLMDYRFDGLRFDAVHAIPDRSFLTELAERVHATVEPGRHVHLVLENEDNRSS
LLTQGFTAQWNDDGHNVLHALLTDESQGYYADYHQDATAKLARFLGEGFIYQGQNNRRGE
ARGEPSAHLSPSSFVLFLQNHDQTGNRAFGDRLVTLTNADALRAATAVLLLSPMVPLLFM
GEEWGSRQPFLFFTSHHGELADAVREGRRSEFAEFSEFADEATRERIPDPNAVATFEQSR
PDFGAQTQPGHAEWHALYRQLLSIRHAEIVPRLPGAAFLGCSVLGEAAVLARWQLGDGCQ
LRLELNLGEQAAPLPASSTAAQLLFASRESDANTPDQLPPRMAKLYLEKAK