Protein Info for Psest_2170 in Pseudomonas stutzeri RCH2

Annotation: 4-alpha-glucanotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 691 TIGR00217: 4-alpha-glucanotransferase" amino acids 117 to 663 (547 residues), 308.4 bits, see alignment E=6e-96 PF02446: Glyco_hydro_77" amino acids 155 to 660 (506 residues), 361.7 bits, see alignment E=3.2e-112

Best Hits

KEGG orthology group: K00705, 4-alpha-glucanotransferase [EC: 2.4.1.25] (inferred from 85% identity to psa:PST_2139)

Predicted SEED Role

"4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GN04 at UniProt or InterPro

Protein Sequence (691 amino acids)

>Psest_2170 4-alpha-glucanotransferase (Pseudomonas stutzeri RCH2)
MSDEALIRLAEAAGLSIDWIDADNREQRVEPAVLREVLACLGLAAETDADIDASLEILAH
KNNHGGVPPLLTCDQHAALDLSAYFPAASRFELQAEDGGVQQGTLDYQARLPAIDAPGYY
RLTIDKHQLTVAIAPLSCPTVAQIAGADAWGLTAQLYSLRRAGDGGLGDTQALESMVSNA
AAHGADALGISPVHAMFSAHINQYSPYSPSSRLFFNVLHAAPGAILGERPLRQAIETCGL
GEELERLERLDLIDWPAVAQSRQRLLRQLFDDFSKGGNPLQVDFDSFRANGGEALENHCR
FEVLHTHLRNAQGHTQHWSDWPSEYRDPASPAVDAFAREHADEVSYHAFGQWLMARGLER
AQVAARSAGMRIGLISDLAVGADGGGSQAWSRQAELLASLSVGAPPDIMNRDGQNWGISA
FSPWGLQQNGFRAYIEMLRANLAHAGGMRIDHVLGLKRLWVVPAGADPKRGVYLNFPFDD
MLRLLCLEAWRHQAVILGEDLGTIPHGLRDVLAARGILGMRVLLFEQHDGHFQTPAQYPA
QALATSTTHDIPTLTGWWRGHDIDWRIKVGQVPESDRDAQWRAREKERAGLNRALCEYSG
KNPDVLLSAEDAVDAAICFLAHTPAPLVLVPVEDALGLEEQTNMPGIVETHPNWRRRYPG
DSATLLDSPASSRRLASFAQARRNHRGAAHR