Protein Info for Psest_2169 in Pseudomonas stutzeri RCH2

Annotation: malto-oligosyltrehalose synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 928 TIGR02401: malto-oligosyltrehalose synthase" amino acids 5 to 925 (921 residues), 941.2 bits, see alignment E=3e-287 PF00128: Alpha-amylase" amino acids 24 to 127 (104 residues), 55 bits, see alignment E=4.3e-19

Best Hits

KEGG orthology group: K06044, (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase [EC: 5.4.99.15] (inferred from 91% identity to psa:PST_2140)

Predicted SEED Role

"Malto-oligosyltrehalose synthase (EC 5.4.99.15)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 5.4.99.15)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIY5 at UniProt or InterPro

Protein Sequence (928 amino acids)

>Psest_2169 malto-oligosyltrehalose synthase (Pseudomonas stutzeri RCH2)
MKDLTATLRLQFHRDFTLDDATALVDYFAELGISHIYASPLLTARPGSMHGYDVIDPTRI
NPELGGEPALHRLVEALRAKGMGLILDIVSNHMAVGGSGNAWWLDVLEWGRRSPYAQFFD
IEWNSPDPLLEGQLLVPFLGSDYGDALQQGTIKLCLDIDNGALYAEHYEHRFPITPPSYG
EVLRAADQQQLRALAQHFDALKTEPAPYQAARLLRAELAEQLQNADTRQALEQALGTYDS
TTDDGFRRLHSLLERQNYRLASWRTAGDDINWRRFFDINELGGLRVERPVVFEETHAKIF
ELIGDGLVDGLRIDHIDGLADPRGYCRRLRRRVDRLNAGRPQDAVQDHVPIYVEKILAGG
ERLHDDWGVDGTTGYEFMNQVSLLQHDPAGEALLCALWSETSGRTTDFLEEARQARQLVL
TGPLAGDFETVAQALLQVARGDVMTRDITLGAIRRALLELIIHFPVYRTYIAAPGRREAD
EPFFQRALDGAKTTLSEADWPLLEHLQRWLGGENLRQLPRNLRKIRRYACTRFQQLTSPA
AAKAVEDTACYRSGILLSRNDVGFDPQHFSAPLQAFHDECLQRAEHFPRNMLTTATHDHK
RGEDTRARMAVISERGDWFAGKVRRWRDMAASRIQQLGDGPAPSPADELMLFQILLGCWP
LDLRADDKAAMEQFCARILQWQEKALREAKLRTTWSDPNGDYEGACRQYVEHLLLASEGA
PLRDEIAAAAAELAPAGALNGLVQSLLRMTTPGVPDLYQGTEYWDFSLVDPDNRRPVDFA
ARQRSLDGSASFDQLLQSWHDGRIKQCVISRTLQLRRRYPRLFSEGRYLPLTVSGEQAEH
LLAFARELDGEWLVVVVPRLVAGLLGDSEIPKVPTERWGDTRIALPEALDGSEFERLFDA
TAVTSQQASLEAARVLDGLPAAVLHTST