Protein Info for Psest_2167 in Pseudomonas stutzeri RCH2

Annotation: glycogen debranching enzyme GlgX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 716 TIGR02100: glycogen debranching enzyme GlgX" amino acids 20 to 709 (690 residues), 1091.2 bits, see alignment E=0 PF02922: CBM_48" amino acids 24 to 112 (89 residues), 63.6 bits, see alignment E=1.7e-21 PF00128: Alpha-amylase" amino acids 205 to 290 (86 residues), 38.9 bits, see alignment E=6.9e-14

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 97% identity to psa:PST_2142)

MetaCyc: 52% identical to isoamylase (Sulfolobus acidocaldarius)
Isoamylase. [EC: 3.2.1.68]

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 3.2.1.68

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GL19 at UniProt or InterPro

Protein Sequence (716 amino acids)

>Psest_2167 glycogen debranching enzyme GlgX (Pseudomonas stutzeri RCH2)
MSKKKPQAPQVMTPSRIREGLPFPLGATWDGLGVNFAIFSAHATKVELCLFDSSGEVELE
RIELPEYTDEIWHGYLPDAHPGLIYGYRVYGPYDPENGHRFNPNKLLIDPYAKQLVGELK
WSEALFGYTIGHPDGDLSFDERDSAPFVPKCKVIDPAFTWGRDHPVQVPWDKTIIYETHV
RGYTMRHPAVADDVRGTFAGFKTPEVIDYIRKLGVSSIELLPIHAFVQDQHLLEKGMANY
WGYNSIAFFAPHPKYLASGKITEFKEMVAHLHNADLEVILDVVYNHTAEGNERGPTLSMR
GIDNASYYRLMPDDKRYYINDSGTGNTLDMSHPCVLQMVTDSLRYWATEMHVDGFRFDLA
TILGREHDGFDERHGFLVACRQDPVLAKTKLISEPWDCGPGGYQVGGFPPGWAEWNDQFR
DTVRSFWKGDDGQLADFASRLTGSGDLFNQRGRRPFSSINFVTAHDGFTLKDLVSYNHKH
NEDNDEGNRDGSDNNLSWNHGVEGHTEDPEINELRYRQMRNFLATLLFSQGTPMIVAGDE
FARTQHGNNNAYCQDSEIGWVNWDISEDSYGLLGFARKLIRMRQRFPMLRRSRFLVGAYN
EELGVKDVTWLAPNAEEMTIEQWEDAHNRCMGMLLDGRAQPTGIRRAGSDATLLIIVNAH
HDLVNFALPEVPQGIYWNRLIDTNHPTARPERFDFNDEYAVTGRSLLLLELIKSDE