Protein Info for HP15_2055 in Marinobacter adhaerens HP15

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 564 TIGR00970: 2-isopropylmalate synthase" amino acids 6 to 547 (542 residues), 708.9 bits, see alignment E=2.3e-217 PF00682: HMGL-like" amino acids 30 to 310 (281 residues), 265.1 bits, see alignment E=1e-82 PF22615: IPMS_D2" amino acids 327 to 390 (64 residues), 113.8 bits, see alignment E=5.5e-37 PF08502: LeuA_dimer" amino acids 422 to 546 (125 residues), 61.5 bits, see alignment E=1.2e-20

Best Hits

Swiss-Prot: 65% identical to LEU1_ALCBS: 2-isopropylmalate synthase (leuA) from Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 89% identity to maq:Maqu_1732)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PRB0 at UniProt or InterPro

Protein Sequence (564 amino acids)

>HP15_2055 2-isopropylmalate synthase (Marinobacter adhaerens HP15)
MAFDHRKYVAFKPVAKTDRRWPDKVIEKAPTWCAVDLRDGNQALVKPMSVAQKTRMFELL
VKLGFKEIEIGFPAASQPDFDFCRKLIEENRIPEDVKIQVLTQARPELIERTYEALAGAR
KAIVHVYNSTSTVQREQVFGLDRDGIRDIAVNGAKLVRDIAARHPETQWTFQYSPESFTG
TELDFAAEVIDAVSEVWRPEEGQPMIINLPATVEMATPNVFADQIEWICDNIQRREHISI
SVHTHNDRGCAVAAAELAVMAGADRVEGTLMGNGERTGNMDLVTMAMNLYSQGIDPTLDL
SGMAEITEVVEACTEISTHPRHPYAGELVFTAFSGSHQDAIRKCLARRKDGETWNVAYLP
IDPFDVGRRYEEVVRINSQSGKGGVAYVLERDYNISLPRWLQIEFSKVVQREAETNGGEI
DSLTIHRLFEDRYLKVHADWALRSYDLHRDEEGVHAEVSVGRDASPVRLDGHGLGAVEAV
SEALEKRFGISIAVEAYDEFALGEGTNANALACIRLTASGMHCSAAALAEDTTSATLQAL
FSAVAQAVGIEMPEKEAEPEAAGA