Protein Info for HP15_2053 in Marinobacter adhaerens HP15

Annotation: PrpE protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16177: ACAS_N
  • amino acids 3 to 55 (53 residues), 59.1 bits, 4.6e-20
• PF00501: AMP-binding
  • amino acids 65 to 446 (382 residues), 283 bits, E=5e-88
• PF13193: AMP-binding_C
  • amino acids 509 to 588 (80 residues), 93.2 bits, E=2.3e-30

Best Hits

Swiss-Prot: 60% identical to Y3568_PSEAE: Uncharacterized protein PA3568 (PA3568) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01908, propionyl-CoA synthetase [EC: 6.2.1.17] (inferred from 86% identity to maq:Maqu_1730)

MetaCyc: 61% identical to propionate--CoA ligase (Ruegeria pomeroyi DSS-3)
Propionate--CoA ligase. [EC: 6.2.1.17]

Predicted SEED Role

"Propionate--CoA ligase (EC 6.2.1.17)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 6.2.1.17)

MetaCyc Pathways

• 2-methylcitrate cycle I (5/5 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• acrylate degradation II (2/3 steps found)
• β-alanine biosynthesis II (4/6 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)

KEGG Metabolic Maps

• Propanoate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.17

Sequence Analysis Tools

See E4PRA8 at UniProt or InterPro

Protein Sequence (624 amino acids)

>HP15_2053 PrpE protein (Marinobacter adhaerens HP15)
MDYHSEFRRSIDKPDEFWREQAEKIDWIEPPKTIWQPTDNGHGQWFPDGTLNTCDVALDA
NIRAGRGDQKALIYDSPVTDTQRSYTYNELTDEVARFAGALKDRGITKGDRVIIYMPMIP
EAVIAMLGCARIGAIHSVVFGGFAAHELAVRIDDAKPKAVITASCGIEVTRVIEYKPLVD
KAIDQASHKPETCIVYQRQQVQAELQACRDHDWNELMETAEPADPVPVKSTDPLYILYTS
GTTGKPKGVVRDNGGHAVALNYSMKLVYDAKPGDVYWAASDVGWVVGHSYIVYAPLFAGC
TTIMYEGKPVKTPDAGAFWRVVQDHKVNMLFTAPTAFRAVRKEDPEADQLSRYDISSLKR
LFLAGERLDPATYEWLKEHTGLPVLDHWWQTETGWAICCNPVGIELMATKPGSATVPSPG
YNVQVVDMNGSQMPAGEQGQIAVKLPLPPGCMMTVWGDDERFRKTYLEPISGFYSSGDGG
FIDDDGYVFVMGRTDDVINVAGHRLSTGEMEEVVASHPAIAECCVVGAHDDMKGQIPIGL
VLIKDGATIDHDELEDELVEMVRDKIGAIACFRRALVVDRLPKTRSGKILRRVIRQIADG
EEYSVPSTIDDPAILEEISEQFRR