Protein Info for HP15_2044 in Marinobacter adhaerens HP15

Annotation: aspartyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 602 TIGR00459: aspartate--tRNA ligase" amino acids 1 to 584 (584 residues), 812.8 bits, see alignment E=7.6e-249 PF01336: tRNA_anti-codon" amino acids 18 to 102 (85 residues), 65.5 bits, see alignment E=6.7e-22 PF00152: tRNA-synt_2" amino acids 120 to 558 (439 residues), 405.7 bits, see alignment E=2.7e-125 PF02938: GAD" amino acids 309 to 408 (100 residues), 95.7 bits, see alignment E=3.3e-31

Best Hits

Swiss-Prot: 94% identical to SYDND_MARHV: Aspartate--tRNA(Asp/Asn) ligase (aspS) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 94% identity to maq:Maqu_1707)

MetaCyc: 60% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23)" (EC 6.1.1.12, EC 6.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PQT8 at UniProt or InterPro

Protein Sequence (602 amino acids)

>HP15_2044 aspartyl-tRNA synthetase (Marinobacter adhaerens HP15)
MRSHYCGGINESHIDQEVTLCGWVHRRRDHGGVIFLDLRDRDGMSQVVVDPDTPESFALA
EKVRSEFVIKVTGRVRRRPAGTENNNMPTGQVELLGKELTILNAAATPPFPLDEHVDVGE
DVRLRYRFVDLRRPEMINRLRFRSRVTSYIRNYLDSNGFMDVETPILTRATPEGARDYLV
PSRTHEGSFFALPQSPQLFKQLLMVSGVDRYYQIAKCFRDEDLRADRQPEFTQVDIEASF
IDEETLMKLNEDMIRALFKDVLEVELPSFPQMPYAEAMQRYGSDKPDLRIPLELLDVNDL
VEGVDFKVFAGPAKDPKGRVAALRVPKGGELTRKQIDDYTKFVGIYGAKGLAYIKVNDLS
KGVEGLQSPIIKFLGDDVALAIMERVGAEDGDIVFFGADKTNVVNEALGALRIKIGHDLD
MLTSEWAPLWVVDFPMFEETPDGGLTAIHHPFTAPSCSPEDLAADPANALSRAYDMVLNG
TELGGGSIRIHDEKMQEAVFRILGIGEEEARAKFGFLLDALKFGCPPHGGLAFGLDRLVM
LMTGASSIRDVIAFPKTQSATCLMTQAPGEVDEKQLRELHIRLRKPSKPVEGNKAEAGEG
NE