Protein Info for Psest_0210 in Pseudomonas stutzeri RCH2

Annotation: Na/Pi-cotransporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 561 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details transmembrane" amino acids 41 to 58 (18 residues), see Phobius details amino acids 65 to 89 (25 residues), see Phobius details amino acids 99 to 121 (23 residues), see Phobius details amino acids 133 to 151 (19 residues), see Phobius details amino acids 171 to 194 (24 residues), see Phobius details amino acids 202 to 226 (25 residues), see Phobius details amino acids 243 to 262 (20 residues), see Phobius details amino acids 275 to 293 (19 residues), see Phobius details TIGR00704: Na/Pi-cotransporter II-related protein" amino acids 1 to 307 (307 residues), 322.4 bits, see alignment E=4e-100 PF02690: Na_Pi_cotrans" amino acids 13 to 147 (135 residues), 117.8 bits, see alignment E=3.9e-38 amino acids 159 to 237 (79 residues), 42 bits, see alignment E=9.9e-15 TIGR01013: sodium-dependent inorganic phosphate (Pi) transporter" amino acids 67 to 520 (454 residues), 333.1 bits, see alignment E=3e-103 PF01895: PhoU" amino acids 339 to 424 (86 residues), 46.3 bits, see alignment E=4.4e-16

Best Hits

Swiss-Prot: 61% identical to YJBB_SALTY: Uncharacterized protein YjbB (yjbB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03324, phosphate:Na+ symporter (inferred from 95% identity to psa:PST_4036)

MetaCyc: 61% identical to putative inorganic phosphate export protein YjbB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-470

Predicted SEED Role

"Sodium-dependent phosphate transporter" in subsystem Phosphate metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GFM1 at UniProt or InterPro

Protein Sequence (561 amino acids)

>Psest_0210 Na/Pi-cotransporter (Pseudomonas stutzeri RCH2)
MLTLLHLLSSIALLVWGTHIVRTGIMRVYGSHLRRMLGQSMGNAPLAFGAGIGVTALVQS
SNATALLAISFVAQGLMALPTALAIMLGADVGTALMARVLTLDLSWLSPLLTLVGVSLFL
SRKQSRSGQIGRVLIGLGLIILALQLIVHAAEPITEARGMQVLFSSLAGDTLLAVLVGAL
FAVLSYSSLAAVLLTSTLAGAGLISLPVALGLVIGANIGSGLLAWFNASLQPASARRVAL
GNLLYKLAGLIVLPFLVPLVAWMDTLGYSAQTQVIGFHLVYNSLRCLILLPTVQPMSRLC
EYLLPERALDNGVAQPRHLDLAALETPSLALANAVRETLRIGDMVELMLARLLEVLREDR
AEPGEEIRRLDDDVDALYSGVKLYLAQMPREDLAEQEGRRWAEIIELAINLEQAGDIIEH
MASKVQNLKTAKRRSFSDSGLEELGQLHGQLTTNLQLGLSVFISGDSHSARQLLQQKRQF
RKLERELAHSHVQRLHQQVVESIETSAAHLELIADMKRLNSLFCSAAYPALEGPGGSKPR
VSTDNPRHVTANEQHLPGKPA