Protein Info for Psest_2109 in Pseudomonas stutzeri RCH2

Annotation: Predicted membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 230 transmembrane" amino acids 15 to 37 (23 residues), see Phobius details amino acids 49 to 68 (20 residues), see Phobius details amino acids 74 to 94 (21 residues), see Phobius details PF20730: YetF_N" amino acids 21 to 88 (68 residues), 27.3 bits, see alignment E=2.5e-10 PF04239: DUF421" amino acids 97 to 164 (68 residues), 45.7 bits, see alignment E=4.6e-16

Best Hits

Swiss-Prot: 54% identical to YCAP_ECOLI: UPF0702 transmembrane protein YcaP (ycaP) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 94% identity to psa:PST_2216)

Predicted SEED Role

"Putative inner membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIT1 at UniProt or InterPro

Protein Sequence (230 amino acids)

>Psest_2109 Predicted membrane protein (Pseudomonas stutzeri RCH2)
MTPFDLQRMLLHEFPLMFVAEVSLRALLAFIAVFVFLKVSGRRGIRQLSVFELVIILTLG
SAAGDVSFYDDVPLLPVAAVFATLLVLYRLTVFFMNRSPRFGVWLEGKPVTIIRDGIYEL
RSLDGLNISADEFFMELRQQGVEHLGQVRLGILENDGNVSLFFHEPEAVRPGLSVLPPEY
RPVFHQVPAAGMYACSRCGFPQALESQQAQSCPRCSNPTWSKALSTLRSR